Research Progress on the Bio-production of Chitin and Chitosan

doi:10.13560/j.cnki.biotech.bull.1985.2018-0633

Abstract

Abstract:

Chitin is another natural biopolymer after cellulose. Chitin and its derivative chitosan have been widely applied in agriculture,medicine,food processing,environmental protection and biotechnology due to the important physicochemical properties,admirable biodegradability,biocompatibility,and no toxicity. However,chitin is hard to be dissolved because of the strong hydrogen bond between chitin molecules,which limits its applications. Chitosan can be obtained from chitin by deacetylation,and the interaction force among chitosan molecules weakens due to the decrease of acetyl groups,which improves the solubility to a certain extent. Meanwhile,chitosan demonstrates more important economic value in drug slow-release,wound healing and other medical aspects,as the presence of a large number of free amino groups. In this paper,the traditional chemical method of preparing chitin,i.e.,acid-base method is described briefly;the method still is of many limitations and disadvantages,such as high energy consumption,high cost and serious environmental pollution,even though it is mature. Based on this,the environmental friendly enzymatic and microbial fermentation methods are summarized,their advantages and disadvantages are discussed respectively,the biological preparation methods of its important derivative chitosan are also summarized. The key issues in chitin/chitosan production from laboratory research to industrialization by biological method are analyzed,and finally the research directions of biological preparation methods for chitin/chitosan are further prospected.

Key words: chitin, chitosan, bio-production

WANG Meng, LI Lan-peng, ZHANG Quan, PENG Shao-zhong, CAO Chang-hai. Research Progress on the Bio-production of Chitin and Chitosan[J]. Biotechnology Bulletin, 2019, 35(4): 213-222.

References

[1] 程倩, 吴薇, 籍保平. 微生物发酵法提取甲壳素的国内外研究进展[J]. 食品科技, 2012, 37(3):40-43.
[2] Muzzarelli RAA, Boudrant J, Meyer D, et al.Current views on fungal chitin/chitosan, human chitinases, food preservation, glucans, pectins and inulin:A tribute to Henri Braconnot, precursor of the carbohydrate polymers science, on the chitin bicentennial[J]. Carbohydrate Poymers, 2012, 87(2):995-1012.
[3] 车小琼, 孙庆申, 赵凯. 甲壳素和壳聚糖作为天然生物高分子材料的研究进展[J]. 高分子通报, 2008, (2):45-49.
[4] 施文涛, 陈鹏. 甲壳素酰化改性的研究进展[J]. 化工进展, 2016, 35(7):2166-2172.
[5] 张步宁, 崔英德, 陈循军, 等. 甲壳素/壳聚糖医用敷料研究进展[J]. 化工进展, 2008, 27(4):520-526.
[6] Cho YW, Cho YN, et al.Water-soluble chitin as a wound healing accelerator[J]. Biomaterials, 1999, 20(22):2139-2145.
[7] Patrulea V, Ostafe V, Borchard G, et al.Chitosan as a starting material for wound healing applications[J]. European Journal of Pharmaceutics and Biopharmaceutics, 2015, 97:417-426.
[8] Muñoz G, Valencia C, Valderruten N, et al.Extraction of chitosan from Aspergillus niger mycelium and synthesis of hydrogels for controlled release of betahistine[J]. Reactive and Functional Polymers, 2015, 91:1-10.
[9] Elieh-Ali-Komi D, Hamblin MR. Chitin and chitosan:production and application of versatile biomedical nanomaterials[J]. International journal of advanced research, 2016, 4(3):411-427.
[10] Aljawish A, Chevalot I, Jasniewski J, et al.Enzymatic synthesis of chitosan derivatives and their potential applications[J]. Journal of Molecular Catalysis B:Enzymatic, 2015, 112:25-39.
[11] Smith A, Perelman M, Hinchcliffe M.Chitosan:a promising safe and immune-enhancing adjuvant for intranasal vaccines[J]. Human vaccines & immunotherapeutics, 2014, 10(3):797-807.
[12] Younes I, Rinaudo M.Chitin and chitosan preparation from marine sources. structure, properties and applications[J]. Marine drugs, 2015, 13(3):1133-1174.
[13] 张述智, 夏伟, 朱绍辉, 等. 从蝇蛆中提取甲壳素的方法:CN103880979A[P].2014-06-25.
[14] 马翠翠, 任健. 蝇蛆壳制备壳聚糖的工艺条件研究[J]. 现代食品科技, 2013, 29(2):331-334.
[15] Gadgey KK, Bahekar A.Study on extraction methods of chitin from crab shell and investigation of its mechanical properities[J]. International Journal of Mechanical Engineering and Technology, 2017, 8(2):220-231.
[16] Truong T, Hausler R, Monette F, et al.Fishery industrial waste valorization for the transformation of chitosan by hydrothermo-chemial method[J]. Revve des Sciences de I’Eau, 2007, 20, 253-262.
[17] 高乐平, 杜予民, 等. 过氧化氢甲壳素脱色反应条件与分子量研究[J]. 武汉大学学报:理学版, 2002, 48(4):453-456.
[18] 陈雪姣, 姜启兴, 等. 南极磷虾甲壳素的脱色工艺研究[J]. 郑州轻工业学院学报(自然科学版), 2014, 29(3):21-24.
[19] 张钟, 高佳欣. 柠檬酸-反相微胶团法提取虾壳中甲壳素的研究[J]. 食品工业科技, 2017, 38(5):269-273.
[20] 孟凡欣, 吴丽艳, 徐盼菊, 等. 虾壳甲壳素提取工艺优化及其辅助降血糖活性研究[J]. 食品研究与开发, 2016, 37(5):95-100.
[21] Gortari MC, Hours RA.Biotechnological processes for chitin recovery out of crustacean waste:a mini-review[J]. Electronic Journal of Biotechnology, 2013, 16(3):14.
[22] Sedaghat F, Yousefzadi M, Toiserkani H, et al.Bioconversion of shrimp waste Penaeus merguiensis using lactic acid fermentation:an alternative procedure for chemical extraction of chitin and chitosan[J]. International Journal of Biological Macromolecules, 2017, 104, 883-888.
[23] Valdez-Peña AU, Espinoza-Perez JD, Sandoval-Fabian GC, et al.Screening of industrial enzymes for deproteinization of shrimp head for chitin recovery[J]. Food Science and Biotechnology, 2010, 19(2):553-557.
[24] Vázquez JA, Noriega D, Ramos P, et al.Optimization of high purity chitin and chitosan production from IIIex argentinus pens by a combination of enzymatic and chemical processess[J]. Carbohydrate Polymers, 2017, 174, 262-272.
[25] 姜红鹰, 周玉玲, 张桂敏, 等. 酶法制备甲壳素系列衍生物的研究进展[J]. 氨基酸和生物资源, 2016, 38(4):71-76.
[26] Kaur S, Dhillon GS.Recent trends in biological extraction of chitin from marine shell wastes:A review[J]. Critical Rev Biotechnol, 2015, 35(1):44-61.
[27] Antunes-Valcareggi SA, Ferreira SRS, Hense H.Enzymatic hydrolysis of blue crab(callinectes sapidus)waste processing to obtain chitin, protein, and astaxanthin-enriched extract[J]. International Journal of Environmental &Agriculture Research, 2017, 3(1):81-92.
[28] Younes I, Ghorbel-Bellaaj O, Nasri R, et al.Chitin and chitosan preparation from shrimp shells using optimized enzymatic deproteinization[J]. Process Biochemistry, 2012, 47(12):2032-2039.
[29] Hamdi M, Hammami A, Hajji S, et al.Chitin extraction from blue crab(Portunus segnis)and shrimp(Penaeus kerathurus)shells using digestive alkaline proteases from P. segnis viscera[J]. International Journal of Biological Macromolecules, 2017, 101:455-463.
[30] Oh YS, Shih L, Tzeng YM, et al.Protease produced by Pseudomonas aeruginosa K-187 and its application in the deproteinization of shrimp and crab shell wastes[J]. Enzyme and Microbial Technology, 2000, 27(1-2):3-10.
[31] Lopes C, Antelo LT, Franco-Uría A, et al.Chitin production from crustacean biomass:Sustainability assessment of chemical and enzymatic processes[J]. Journal of Cleaner Production, 2018, 172:4140-4151.
[32] Arbia W, Arbia L, Adour L, et al.Chitin extraction from crustacean shells using biological methods-A review[J]. Food Technology & Biotechnology, 2013, 51(1):12-25.
[33] Nguyen TT, Barber AR, Smith P, et al.Appliaction and optimization of the highly efficient and environmentally-friendly microwave-intensified lactic acid demineralization of deproteinized Rock lobster shells(Jasus edwardsii)for chitin production[J]. Food and Bioproducts Processing, 2017, 102:367-374.
[34] Ploydee E, Chaiyanan, S.Production of high viscosity chitosan from biologically purified chitin isoated by microbial fermentation and deproteinization[J]. International Journal of Polymer Science, 2014, 2014(5):1-8.
[35] Neves AC, Zanette C, et al.Optimization of lactic fermentation for extraction of chitin from freshwater shrimp waste[J]. Acta Scientiarum Technology, 2017, 39(2):125-133.
[36] Ghorbel-Bellaaj O, Hmidet N, Jellouli K, et al.Shrimp waste fermentation with Pseudomonas aeruginosa A2:optimization of chitin extraction conditions through Plackett-Burmanand response surface methodology approaches[J]. International Journal of biological Macromolecules, 2011, 48(4):596-602.
[37] Choorit W, Patthanamanee W, Manurakchinakorn S.Use of response surface method for the determination of demineralization efficiency in fermented shrimp shells[J]. Bioresour Technol, 2008, 99(14):6168-6173.
[38] Ghorbel-Bellaaj O, Younes I, Maalej H, et al.Chitin extraction from shrimp shell waste using Bacillus bacteria[J]. International Journal of Biological Macromolecules, 2012, 51(5):1196-1201.
[39] Zhang H, Yu H, Qian Y, et al.Production of chitin and chitosan using successive three-step microbial fermentation[J]. Journal of Polymer Materials, 2017, 34(1):123-127.
[40] Aytekin O, Elibol M.Cocultivation of Lactococcus lactis and Teredinobacter turnirae for biological chitin extraction from prawn waste[J]. Bioprocess and Biosystems Engineering, 2010, 33(3):393-399.
[41] Zhang HC, Jin YF, Deng Y, et al.Production of chitin from shrimp shell powders using Serratia marcescens B742 and Lactobacillus plantarum ATCC 8014 successive two-step fermentation[J]. Carbohydrate Research, 2012, 362(2):13-20.
[42] Duan S, Zhang YX, Lu TT, et al.Shrimp waste fermentation using symbiotic lactic acid bacteria[J]. Advanced Materials Research, 2011, 194:2156-2163.
[43] Liu P, Liu SS, Guo N, et al.Cofermentation of Bacillus licheniformis and Gluconobacter oxydans for chitin extraction from shrimp waste[J]. Biochemical Engineering Journal, 2014, 91:10-15.
[44] 段人钰. 一种利用虾废料同时制取虾青素、壳聚糖的方法:CN106520984A[P].2017-03-22.
[45] Hajji S, Younes I, Ghorbel-Bellaaj O, et al.Structural differences between chitin and chitosan extracted from three different marine sources[J]. International Journal of Biological Macromolecules, 2014, 65:298-306.
[46] Chang KLB, Tsai G, Lee J, et al.Heterogeneous N-deacetylation of chitin in alkaline soution[J]. Carbohyd Research, 1997, 303(3):327-332.
[47] Berger J, Reist M, Chenite A, et al.Pseudo-thermosetting chitosan hydrogels for biomedical application[J]. International Journal of Pharmaceutics, 2005, 288(2):197-206.
[48] Kaczmarek MB, Struszczyk-Swita K, et al.Isolation, molecular coning and characterization of two genes coding chitin deacetylase from Mucor circinelloides IBT-83[J]. Progress of Chemistry & Application of Chitin & Its Derivatives, 2016, 21:93-103.
[49] Liu Z, Gay LM, Tuveng TR, et al.Structure and function of a broad-specificity chitin deactylase from Aspergillus nidulans FGSC A4[J]. Scientific Reports, 2017, 7(1):1746.
[50] Ramos-Puebla A, Santiago CD, Trombotto S, et al.Addition of abscisic acid increases the production of chitin deacetylase by Colletotrichum gloeosporioides in submerged culture[J]. Process Biochemistry, 2016, 51(8):959-966.
[51] Blair DE, Hekmat O, Schüttelkopf AW, et al.Structure and mecha-nism of chitin deacetylase from the fungal pathogen Colletotrichum lindemuthianum[J]. Biochemistry, 2006, 45(31):9416-9426.
[52] Martinou A, Bouriotis V, et al.Mode of action of chitin deacetylase from Mucor rouxii on partially N-acetylated chitosans[J]. Carbohydrate Research, 1998, 311(1-2):71-78.
[53] 王皓, 吴丽, 朱小花, 等. 甲壳素脱乙酰酶的研究概况及展望[J]. 中国生物工程杂志, 2015, 35(1):96-103.
[54] Tsigos I, Bouriotis V.Purification and characterization of chitin deacetylase from Colletotrichum lindemuthianum[J]. J Biol Chem, 1995, 270(44):26286-26291.
[55] Kang LX, Liang YX, Ma LX.Novel characteristics of chitin deacetylase from Colletotrichum lindemuthianum:Production of fully acetylated chitooligomers, and hydrolysis of deacetylated chitooligomers[J]. Process Biochemistry, 2014, 49(11):1936-1940.
[56] 窦勇, 胡佩红. 超声协同CDA酶法制备龙虾壳聚糖[J]. 食品与发酵工业, 2014, 40(11):127-131.
[57] Aspras I, Kamińska M, Karzyński K, et al.The influence of selected ionic liquids on activity of chitin deacetylase[J]. Chemical and Process Engineering, 2016, 37(1):77-82.
[58] Jaworska MM.Kinetics of enzymatic deacetylation of chitosan[J]. Cellulose, 2012, 19(2):363-369.
[59] Ghormade V, Pathan EK, Deshpande MV.Can fungi compete with marine sources for chitosan production?[J]. International Journal of Biological Macromolecules, 2017, 104:1415-1421.
[60] Dhillon GS, Kaur S, Sarma SJ, et al.Integrated process for fungal citric acid fermentation using apple processing wastes and sequential extraction of chitosan from waste stream[J]. Industrial Crops and Products, 2013, 50:346-351.
[61] Pochanavanich P, Suntornsuk W.Fungal chitosan production and its characterization[J]. Letters in Applied Microbiology, 2002, 35(1):17-21.
[62] Rane KD, Hoover DG.Production of chitosan by fungi[J]. Food biotechnology, 1993, 7(1):11-33.
[63] Muzzarelli RAA, Ilari P, Tarsi R, et al.Chitosan from Absidia coerulea[J]. Carbohydrate Polymers, 1994, 25(1):45-50.
[64] Kim WJ, Lee WG, Theodore K, et al.Optimization of culture conditions and continuous production of chitosan by the fungi, Absidia coerulea[J]. Biotechnology and Bioprocess Engineering, 2001, 6(1):6-10.
[65] Abdel-Gawad KM, Hifney AF, et al.Technology optimization of chitosan production from Aspergillus niger biomass and its functional activities[J]. Food Hydrocolloids, 2017, 63:593-601.
[66] Tasar OC, Erdal S, Taskin M.Chitosan production by psychrotole- rant Rhizopus oryzae in non-sterile open fermentation conditions[J]. International Journal of Biological Macromolecules, 2016, 89:428-433.
[67] Doiphode N, Rajamohanan PR, et al.Chitosan production using a dimorphic zygomycetous fungus Benjaminiella poitrasii:role of chitin deacetylase[J]. Asian Chitin J, 2009, 5:19-26.
[68] Amorim RVDS, Pedrosa RP, Fukushima K, et al.Alternative carbon sources from sugar cane process for submerged cultivation of Cunninghamella bertholletiae to produce chitosan[J]. Food Technology & Biotechnology, 2006, 44(4):519-523.
[69] Amorim RVS, Souza W, Fukushima K, et al.Faster chitosan production by mucoralean strains in submerged culture[J]. Brazilian Journal of Microbiology, 2001, 32(1):20-23.
[70] Vendruscolo F, Ninow JL.Apple pomace as a substrate for fungal chitosan production in an airlift bioreactor[J]. Biocatalysis and Agricultural Biotechnology, 2014, 3(4):338-342.
[71] Cardoso A, Lins CIM, dos Santos E R, et al. Microbial enhance of chitosan production by Rhizopus arrhizus using agroindustrial substrates[J]. Molecules, 2012, 17(5):4904-4914.
[72] Gharieb MM, El-Sabbagh SM, Shalaby MA, et al.Production of chitosan from different species of Zygomycetes and its antimicrobial activity[J]. Int. J. Sci. Eng. Res, 2015, 6:123-130.
[73] Yang L, Li X, Lai C, et al.Fungal chitosan production using xylose rich of corn stover prehydrolysate by Rhizopus oryzae[J]. Biotechnology & Biotechnological Equipment, 2017, 31(6):1160-1166.