Identification and Analysis of Biocontrol Proteins from a Strain of Bacillus amyloliquefaciens

doi:10.13560/j.cnki.biotech.bull.1985.2017-0630

Abstract

Abstract: It is aimed at the identification of protein composition with biocontrol function in the secretory protein while a strain of being antagonistic grape downy mildew,Bacillus amyloliquefaciens,was cultured in NA liquid medium,which was carried out by protein profile. The proteomics of the identified proteins was analyzed by gene ontology and KEGG pathway for screening the proteins related to biocontrol. The results showed that 53 proteins were identified from the secreted proteins. Their relative molecular weights were mostly（79.24%）in 0-100. The proteins involved in the processes of carbohydrate metabolism,energy metabolism,lipid metabolism,amino acid metabolism,bio-defense,etc. They also were the cellular components in structural composition. Six proteins involved in the interaction of plant and pathogen were discovered,and among them,two belonged to the aminopeptidase family,and four were involved in the biodegradation of chitin.

Key words: Bacillus amyloliquefaciens, biocontrol, gene ontology, aminopeptidase, chitinase

GUO Ji-ping, MA Guang, WANG Zhi-jie, QI Shan-hou, WANG Bao-mei, SU Chang-qing. Identification and Analysis of Biocontrol Proteins from a Strain of Bacillus amyloliquefaciens[J]. Biotechnology Bulletin, 2018, 34(1): 202-207.

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