Effects of Exogenous Silicon on Growth,Leaf Photosynthesis and Physiological Indexes of Tobacco Seedlings Under Drought Stress

doi:10.13560/j.cnki.biotech.bull.1985.2018-0691

Abstract

Abstract: The effects of different concentrations of exogenous silicon（0,0.5,and 1.0 mmol/L）on the growth,Leaf photosynthetic characteristics and physiological indexes of tobacco seedlings under drought stress（10% PEG and 20% PEG）were studied using nutrient solution culture method. The results showed that drought stress severely inhibited the growth and photosynthesis of tobacco seedlings,and decreased membrane stability and induced oxidative stress response. Application of different concentrations of exogenous silicon effectively improved the growth of tobacco seedlings under drought stress,showing that the growth indices such as plant height,leaf area,root volume,dry weight of root and shoot increased;the contents of chlorophyll a,chlorophyll b,chlorophyll a+b,and carotenoids increased;the net photosynthetic rate（P_n）and transpiration rate（T_r）and stomatal conductance（G_s）significantly increased,while intercellular CO₂concentration（C_i）decreased;MDA content of membrane peroxidation product significantly reduced;and water content of leaves,membrane stability coefficient,and osmotic adjustment substance（proline and soluble sugar）content significantly increased. The activities of antioxidative enzymes such as SOD,POD and CAT increased. The 1.0 mmol/L Si treatment significantly affected the growth and physiological characteristics of tobacco seedlings under drought stress better than 0.5 mmol/L Si treatment. All above results revealed that the application of exogenous silicon may improve the photosynthesis,antioxidation and osmotic adjustment ability of tobacco seedlings under drought stress,relieve drought damage on tobacco seedlings,and promote its growth.

Key words: exogenous silicon, drought, tobacco seedlings, leaf photosynthesis, physiological indicators

ZHANG Huan-wei, CHEN Biao, WEN Xin-yi, ZHANG Jie, WANG Xiao-dong, LI Ji-wei, XU Zi-cheng, HUANG Wu-xing. Effects of Exogenous Silicon on Growth,Leaf Photosynthesis and Physiological Indexes of Tobacco Seedlings Under Drought Stress[J]. Biotechnology Bulletin, 2019, 35(1): 17-26.

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