Fermentation Condition and Phosphate-dissolving Capacity of Phosphate-solubilizing Bacterium

doi:10.13560/j.cnki.biotech.bull.1985.2018-0733

Abstract

Abstract: The aim of the present work is to isolate a highly effective phosphate-solubilizing strain PS-3 for improving phosphorus supply of saline-alkali soil in Xinjiang. The single factor experiment and the orthogonal experiment were used to analyze the fermentation conditions and the phosphate-dissolving capability of strain PS-3. The results showed that the appropriate fermentation conditions of the strain PS-3 were temperature 35℃,pH 7 and salt concentration 1%. The order of the strain utilizing carbon and nitrogen was glucose > maltose>fructose>sucrose>lactose,and ammonium nitrate>sodium nitrate>ammonium chloride>ammonium sulfate>urea,respectively. The optimal conditions for the solubilization of strain PS-3 were 2% glucose,0.01% ammonium nitrate,temperature 35℃,initial pH 7.5,salt content 2 g/L,and inoculation amount 4%. The phosphate-solubilizing capability reached the level of 1 002.95 mg/L under this condition. The strain PS-3 is tolerant to salt stress and has desired phosphate-solubilizing capacity,and thus has a better potential for microbial inoculants with phosphorus-solubilizing ability.

Key words: phosphate-solubilizing bacterium, fermentation condition, phosphate-solubilizing capability

HAN Lei, YANG Le, TANG Jin-ming, ZHANG Ya-qian. Fermentation Condition and Phosphate-dissolving Capacity of Phosphate-solubilizing Bacterium[J]. Biotechnology Bulletin, 2019, 35(1): 98-104.

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