Abstract: In the bioremediation of heavy metal pollution,the potential of algae absorbing heavy metals has attracted the attentions of many researchers. In order to investigate the difference in adsorption and tolerance of photobiont isolated from lichen body to heavy metals Cu²⁺ and Zn²⁺,2 photobionts（B and P.E）were used as research materials,Evan’s blue staining method,BCO and dithizone spectrophotometric method were to determine the cell viability of photobiont,as well as the Cu²⁺ and Zn²⁺ content in culture medium and algae. The results showed that the cell viability and heavy metal adsorption of the 2 photobionts differed under different concentrations of Cu²⁺ and Zn²⁺. Tolerance to Cu²⁺ stress and adsorption was as following P.E > B. Under Zn²⁺ stress,the tolerance after 9 d of culture was P.E > B;as the culture time prolonged,the cell viability of P.E decreased sharply and was lower than that of B,but the adsorption rate was still at P.E > B. Moreover,the tolerance and adsorption of 2 photobionts to Zn²⁺ stress were significantly higher than Cu²⁺ stress. The study found that the adsorptions of 2 photobiont to Zn²⁺ stress were significantly higher than that to Cu²⁺ stress. The study found that the two algae species of Peltigera lichen,special organisms from the symbiosis of bacteria and algae-,had higher tolerance and adsorption to Cu²⁺ and Zn²⁺ stress than some freely-growing algae.

Key words: photobiont, vitality, heavy metal stress, tolerance, adsorption