Construction of An Oxidation Pathway of Xylonic Acid in Bacillus subtilis for Production of Glycolic Acid

doi:10.13560/j.cnki.biotech.bull.1985.2019-0038

Abstract

Abstract: It is aimed that a strain of a food-safety bacterium producing glycolic acid can be obtained by over-expressing foreign genes. An over-expression system comprising of xylonate degradation pathway from Escherichia coli was constructed and introduced into Bacillus subtilis（A164S）,glycolic acid was detected as the main product in the broth of recombinant B. subtilis,using high performance liquid chromatography（HPLC）,and the conversion rate of xylonite to glycolic acid reached 42.54%. Meanwhile,Bacillus subtilis,because of its own nonspecific aldolase and glyoxal dehydrogenase,obtained the ability to biologically transform xylic acid under the condition that only xylose dehydratase YjhG was expressed. In conclusion,a strain of B. subtilis producing glycolic acid from xylic acid is successfully constructed and the possible existence of YjhH isozyme is uncovered.

Key words: Bacillus subtilis, glycolic acid, xylonic acid, xylonate dehydratase

LING Xie, JI Ming-hua, DUAN Hai-yan, SHI Ji-ping, SUN Jun-song. Construction of An Oxidation Pathway of Xylonic Acid in Bacillus subtilis for Production of Glycolic Acid[J]. Biotechnology Bulletin, 2019, 35(6): 76-82.

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