Cultivable Myxobacteria and Their Antibiotic Activities in the Hulun Buir Area of Inner Mongolia

doi:10.13560/j.cnki.biotech.bull.1985.2019-0210

Abstract

Abstract: Total 20 soil samples were collected from Hulun Buir,Inner Mongolia and their environmental parameters were measured. Myxobacteria were isolated from 20 soil samples and then purified and identified by Escherichia coli-inducing method,the rabbit dung pellets-inducing method and filter paper-inducting method. Then the antibiotic activities of the myxobacterial strains were determined by the plate confrontation method,and the results were analyzed by statistical methods. The results showed that the soil nutrient content was unbalanced in Hulun Buir,the soil samples were generally acidic,and the soil water content was low. A total of 41 strains of myxobacteria were obtained,belonging to Myxococcus,Corallococcus,Cystobacter and Archangium;and the dominant one was Myxococcus. The strains showed rich diversity in black soil and grassland. There was no significant correlation between the strains and environmental factors. All the strains presented activity against E. coli and Phytophthora infestans,3 strains inhibited Staphylococcus aureus,but they show no inhibitory effect on Bacillus subtilis,Saccharomyces cerevisiae,Sclerotinia sclerotiorum,Rhizoctonia solanikuhn,and Fusarium oxysporum. In conclusion,the cultivable myxobacteria are abundant in Hulun Buir,and all of them demonstrated obvious activity against P. infestans,providing basic data for the research and development of myxobacteria resources in Hulun Buir,Inner Mongolia.

Key words: Hulun Buir, myxobacteria, environmental parameters, isolation and identification, antibiotic activity, correlation analysis

WANG Xue-han, MA Qiang, TIAN Yuan, HU Jing, LIU Hui-rong. Cultivable Myxobacteria and Their Antibiotic Activities in the Hulun Buir Area of Inner Mongolia[J]. Biotechnology Bulletin, 2019, 35(9): 224-233.

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