Effects of Exogenous Melatonine on the Physiological Characteristics of Peony Seedlings Under Drought Stress

doi:10.13560/j.cnki.biotech.bull.1985.2020-0055

Abstract

Abstract: To further explore the effects of exogenous melatonine（MT）in strengthening the ability of peony seedlings resisting drought and its physiological mechanisms,Paeonia ostii（Fengdan）seedlings were selected to study the effects of different concentrations（50,100,and 200 μmol/L）of exogenous MT on their growth and physiological characteristics under drought stress. The results showed that the growth and photosynthesis of the peony seedlings were significantly inhibited under drought stress,and lipid peroxidation and injury index of leaf cells increased,leading to oxidative stress and osmotic response. The damages caused by drought stress on the peony seedlings treated with the exogenous MT were alleviated,and specifically demonstrated as the mass of roots,stems,leaves and total dry matter increased,the degradation of photosynthetic pigments such as chlorophyll a,chlorophyll b and carotenoids reduced,and photosynthesis and PSⅡelectron transport ability improved,MDA,H₂O₂,O₂^- content and cell membrane damage index reduced,the activity of antioxidant enzymes such as SOD,CAT,APX and GR increased to reduce lipid peroxidation,and thus the accumulation of osmotic adjustment substances was improved. In general,100 μmol/L MT is considered to be the optimal concentration on improving the drought resistance of the peony seedlings. Pretreatment with exogenous MT may effectively enhance the photosynthetic fluorescence characteristics,antioxidant capacity and osmotic adjustment ability,thus promote the growth of peony seedlings under drought stress.

Key words: melatonin, drought stress, peony seedling, photosynthetic fluorescence characteristics, anti-oxidation, osmotic adjustment

FAN Hai-xia, ZHAO Sa, XIN Guo-qi, LI Jing. Effects of Exogenous Melatonine on the Physiological Characteristics of Peony Seedlings Under Drought Stress[J]. Biotechnology Bulletin, 2020, 36(6): 63-72.

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