Identification and Biocontrol Activity Analysis of Bacillus sp. BS-6 Based on Genome-wide Data

doi:10.13560/j.cnki.biotech.bull.1985.2019-0394

Abstract

Abstract: Based on the genome-wide data,the taxonomic status of Bacillus sp. BS-6 isolated from soil was clarified. The antagonistic effect of Bacillus sp. BS-6 on multiple plant pathogens was validated to uncover its potential biocontrol functions. Phylogenetic trees were constructed by 16S rRNA and gyrA gene sequences analysis to identify Bacillus sp. BS-6. Moreover,whole-genome sequencing analysis was also employed to determine the taxonomic status of strain BS-6. Plate isolate-antagonistic experiments were applied to explore the antagonistic effect of strain BS-6 on Fusarium oxysporum,Rhizoctonia solani and Botryospuaeria berengeriana. antiSMASH was used to analyze and predict the antibiotic genes of strain BS-6 among secondary metabolite biosynthesis gene clusters and to explore its biocontrol potential. According to the results of phylogenetic trees,average nucleotide identity and digital DNA-DNA hybridization,BS-6 was characterized as Bacillus subtilis. The BS-6,with 7 important or unique secondary metabolite gene clusters of Bacillus and 4 unknown function secondary metabolite gene clusters in genome,exhibited a fine ability of inhibiting the growth of fungi. Bacillus subtilis BS-6 has strong antagonistic ability against plant pathogens and promising application prospects in agriculture.

Key words: Bacillus subtilis, antagonistic ability, genome, gene clusters

QI Jia-ming, SUN Shan-shan, ZHANG Dong-xu, XU Zhi-wen, XU Yan-ping. Identification and Biocontrol Activity Analysis of Bacillus sp. BS-6 Based on Genome-wide Data[J]. Biotechnology Bulletin, 2019, 35(10): 111-118.

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