[1] Bertolini LR, Meade H, Lazzarotto CR, et al.The transgenic animal platform for biopharmaceutical production[J]. Transgenic Res, 2016, 25(3):329-343. [2] de la Peña JB, Dela Peña IJ, Custodio RJ, et al. Exploring the validity of proposed transgenic animal models of attention-deficit hyperactivity disorder(ADHD)[J]. Mol Neurobiol, 2018, 55(5):3739-3754. [3] Gu H, Li J, Ying F, et al.Analysis of differential gene expression of the transgenic pig with overexpression of PGC1α in muscle[J]. Mol Biol Rep, 2019, doi:10. 1007/s11033-019-04805-8. [4] Afolabi AS, Worland B, Snape J, et al.Novel pGreen/pSoup dual-binary vector system in multiple T-DNA co-cultivation as a method of producing marker-free(clean gene)transgenic rice(Oriza sativa L)plant[J]. Afr J Biotechnol, 2005, 4(6):531-540. [5] Brakta S, Mas A, Al-Hendy A.The ontogeny of myometrial stem cells in OCT4-GFP transgenic mouse model[J]. Stem Cell Res Ther, 2018, 9(1):333. [6] Pham CT, Macivor DM, Hug BA, et al.Long-range disruption of gene expression by a selectable marker cassette[J]. Proceedings of the National Academy of Sciences of the United States of America, 1996, 93(23):13090-13095. [7] Rucker EB, Dierisseau P, Wagner KU, et al.Bcl-x and Bax regulate mouse primordial germ cell survival and apoptosis during embryogenesis[J]. Molecular Endocrinology, 2000, 14(7):1038-1052. [8] McLellan MA, Rosenthal NA, Pinto AR. Cre-loxP-mediated recom-bination:general principles and experimental considerations[J]. Curr Protoc Mouse Biol, 2017, 7(1):1-12. [9] Xu C, Wang K, Ding YH, et al.Claudin-7 gene knockout causes destruction of intestinal structure and animal death in mice[J]. World J Gastroenterol, 2019, 25(5):584-599. [10] Li Y, Chen S, Fang J, et al.Construction of an attenuated Tian Tan vaccinia virus strain by deletion of TA35R and TJ2R genes[J]. Virus Res, 2018, 256:192-200. [11] Ma Y, Yu L, Pan S, et al.CRISPR/Cas9-mediated targeting of the Rosa26 locus produces Cre reporter rat strains for monitoring Cre-loxP-mediated lineage tracing[J]. FEBS J, 2017, 284(19):3262-3277. [12] Zhong Y, Huang W, Du J, et al.Improved Tol2-mediated enhancer trap identifies weakly expressed genes during liver and β cell development and regeneration in zebrafish[J]. J Biol Chem, 2019, 294(3):932-940. [13] 兰翀, 任丽娜, 吴敏, 等. 利用Cre/LoxP系统删除转基因山羊体内的选择标记基因[J]. 生物工程学报, 2013, 29(12):1847-1854. [14] 吴慧. Cre/loxp重组系统介导转基因绒山羊标记基因删除的研究[D]. 呼和浩特:内蒙古大学, 2014. [15] Song SZ, Ge X, Cheng YB, et al.High-level expression of a novel recombinant human plasminogen activator(rhPA)in the milk of transgenic rabbits and its thrombolytic bioactivity in vitro[J]. Mol Biol Rep, 2016, 43:775-783. [16] 宋绍征. 转基因兔乳腺特异性表达重组人纤溶酶原激活剂(rhPA)及其药效学研究[D]. 扬州:扬州大学, 2015. [17] Song SZ, He ZY, Mei JY, et al.Affinity purification of recombinant human plasminogen activator from transgenic rabbit milk using a novel polyolresponsive monoclonal antibody[J]. Tropical Journal of Pharmaceutical Research, 2016, 15(5):905-911. [18] Nguyen HN, Ishidoh KI, Kinoshita H, et al.Targeted cloning of a large gene cluster from Lecanicillium genome by Cre/loxP based method[J]. J Microbiol Methods, 2018, 150:47-54. [19] 李秀, 高慧英, 杨小玉, 等. 基于LoxP-Cre 系统的 Ronin 基因造血系统条件性敲除小鼠模型的构建及鉴定[J]. 军事医学, 2018, 42(9):657-661. [20] Dillen W, De Clercq J, Kapila J, et al.The effect of temperature on Agrobacterium tumefaciens-mediated gene transfer to plants[J]. Plant Journal 1997, 12(6):1459-1463. [21] Chang JC, Lee SJ, Kim JS, et al.Transient expression of foreign genes in insect cells(sf9)for protein functional assay[J]. J Vis Exp, 2018(132):e56693. [22] Roberts R, Zhang J, Mihelich N, et al.Manipulation of oat protoplasts for transient expression assays[J]. Methods Mol Biol, 2017, 1536:55-70. [23] Liu J, Luo Y, Liu QQ, et al.Production of cloned embryos from caprine mammary epithelial cells expressing recombinant human beta-defensin-3[J]. Theriogenology, 2013, 79(4):660-666. [24] Xu HF, Luo J, Zhang XY, et al.Activation of liver X receptor promotes fatty acid synthesis in goat mammary epithelial cells via modulation of SREBP1 expression[J]. J Dairy Sci, 2019, 102(4):3544-3555. [25] 袁玉国, 丁国梁, 安礼友, 等. 以经过转染的乳腺上皮细胞生产克隆羊[J]. 生物工程学报, 2009, 25(8):1138-1143. [26] Cui WT, Ren LM, Hou J, et al.A model study:simulation of marker gene deletion and target gene replacement mediated by Cre/loxp system in cell line[J]. Progress in Biochemistry and Biophysics, 2008, 35(6):650-660. [27] Lv Y, Edwards H, Zhou J, et al.Combining 26s rDNA and the Cre-loxP system for iterative gene integration and efficient marker curation in yarrowia lipolytica[J]. ACS Synth Biol, 2019, 8(3):568-576. |