Determination the Content of Total Flavonoids and Hypericin in Callus from Hypericum attenuatum Choisy

doi:10.13560/j.cnki.biotech.bull.1985.2015.01.015

Abstract

Abstract: With fresh leaves as test material, this study established a relatively stable system of callus culture for Hypericum attenuatum Choisy, and determined the content of total flavonoids and hypericin in callus. With MS as the basic culture medium, the effects of different hormone combinations were investigated on callus induction. The results showed that the optimum culture media for callus induction was MS + 2，4-D 4.0 mg/L + 6-BA 0.2 mg/L. The total flavonoids and hypericin of callus were extracted using ultrasonic method, respectively, using UV spectrophotometry and HPLC method for determination total flavonoids content and hypericin content in callus. The results showed that total flavonoids was 27.99 mg/g, and hypericin content was 0.515 mg/g in dry callus.

Key words: Hypericum attenuatum Choisy, callus, induction, total flavonoids, hypericin

Liu Xiaodan, Zhang Keqin, Liu Lian, Li Wenchang. Determination the Content of Total Flavonoids and Hypericin in Callus from Hypericum attenuatum Choisy[J]. Biotechnology Bulletin, 2015, 31(1): 98-103.

share this article

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://biotech.aiijournal.com/EN/10.13560/j.cnki.biotech.bull.1985.2015.01.015

https://biotech.aiijournal.com/EN/Y2015/V31/I1/98

References

[1] 陈茂霞, 朱慧. 贯叶金丝桃的化学成分与药理作用研究进展[J]. 中药研究与信息, 2001, 3（10）：24-27.
[2] 余爱农, 龚发俊, 马济美. 金丝桃素研究新进展[J]. 湖北民族学院学报：自然科学版, 2003, 21（1）：44-47.
[3] Agostinis P, Vantieghem A, Merlevede W, et al. Hypericin in cancer treatment：more light on the way[J]. Int J Biochem Cell Biol, 2002, 34（3）：221.
[4] Veronika B, Guido J, Adolf N, et al. Flavonoid from Hypericum perforatum show antidepressant activity in the Forced Swimming Test[J]. Planta Med, 2000, 66：3.
[5] 张喜. 乌腺金丝桃中金丝桃素的含量测定及提取纯化金丝桃素的工艺研究[D] . 长春: 吉林大学, 2011.
[6] 王晓菊, 张立伟. 贯叶连翘中金丝桃素分离工艺的研究[J]. 山西大学学报：自然科学版, 2005, 28（1）：75-77.
[7] 李晓坤, 于雷, 郝鹏飞, 等. 金丝桃素的酶提取工艺优选[J]. 中国实验方剂学杂志, 2012, 18（8）：46-49.
[8] 贺建国, 赵晶, 王金委. HPLC法测定滇西产金丝桃属植物中金丝桃素的含量[J]. 西南国防医药, 2002, 12（1）：23-25.
[9] 胡君萍, 杨建华, 马成, 堵年生. HPLC测定新疆贯叶金丝桃中金丝桃素的含量[J]. 华西药学杂志, 2003, 18（5）：369-371.
[10] 郑炜, 连树林, 孙国辉, 等. HPLC测定乌腺金丝桃中金丝桃素的含量[J]. 吉林中医药, 2010, 30（11）：999-1000.
[11] 董建勇, 贾忠建. 赶山鞭中黄酮类化学成分研究[J]. 中国药学杂志, 2005, （12）：897-899.
[12] 张克勤, 薛晓丽, 孔令瑶, 等 . 长柱金丝桃中金丝桃素的含量变化[J]. 中国药学杂志, 2011, 46（3）：174-176.
[13] 李冀, 石鑫, 高彦宇. 乌腺金丝桃抗抑郁作用的药理研究[J]. 中医药信息, 2012（2）：16-17.
[14] 李冀, 闫东, 毕珺辉. 乌腺金丝桃正丁醇萃取物对氯化钙诱发大鼠快速性心律失常的影响[J]. 中医药信息, 2012（4）：144-145.
[15] Qian J, Wu J, Lu Y. Preparation of a polyclonal antibody against hy-pericin synthase and localization of the enzyme in red-pigmented Hypericum perforatum L. plantlets[J]. Acta Biochimica Polonica, 2012, 59（4）：639-645.
[16] Olivo M, Fu CY, Raghavan V, et al. New frontier in hypericin medi-ated diagnosis of cancer with current optical technologies[J]. Ann Biomed Eng, 2012, 40（2）：460-473.
[17] Barathan M, Mariappan V, Shankar EM, et al. Hypericin-photody-namic therapy leads to interleukin-6 secretion by HepG2 cells and their apoptosis via recruitment of BH3 interacting-domain death agonist and caspases[J]. Cell Death Dis, 2013, 4：e697.
[18] 尹兴斌, 曲昌海, 张晓燕, 等. 贯叶金丝桃中金丝桃苷在比格犬体内的药代动力学研究[J]. 中国实验方剂学杂志, 2013, 19（2）：140-143.
[19] Cardoso MA, Oliveira DE. Tissue culture of Hypericum brasiliense Choisy：Shoot multiplication and callus induction[J]. Plant Cell, Tissue and Organ Culture, 1996, 44（2）：91-94.
[20] 丁如贤, 许铁峰, 张汉明. 贯叶连翘的组织培养和快速繁殖[J]. 第二军医大学学报, 2000, 21（10）：904-906.
[21] Pretto FR, Santarem ER. Callus formation and plant regeneration from Hypericum perforatum leaves[J]. Plant Cell, Tissue and Organ Culture, 2000, 62（2）：107-113.
[22] 宋馨, 祝建, 吕洪飞, 李飞. 贯叶金丝桃愈伤组织分化及二慈酮类物质的积累[J]. 中草药, 2006, 37：305-308.
[23] 林华, 王永清, 林静. 川渝金丝桃的组织培养[J]. 植物生理学通讯, 2007, 43（4）：735-735.
[24] 王海菲. 艳果金丝桃再生和遗传转化体系的研究[D] . 武汉: 华中农业大学, 2011.
[25] Bais HP, Vepachedu R, Lawrence CB, et al. Molecular and bioche-mical characterzation of an enyzme responsible for the formation of hypericin in St. John’s Wort（Hypericum perforatum L.）[J]. The Journal of Biological Chemistry, 2003, 278：32413-32422.
[26] 王力. 金丝桃属合成酶的基因克隆、表达及其酶学性质研究[D] . 北京: 中国农业大学, 2005.
[27] Kartning TH, Gobel I. Determination of hypericin and paeudohype-ricin by thin-layer chromatography-densitometry[J]. Journal of Chromatography A, 1992, 609（1-2）：423-426.
[28] 邵敏敏. 贯叶连翘和小连翘的组织培养及分泌结构与金丝桃素类物质含量的相关性研究[D] . 金华：浙江师范大学, 2008.
[29] 于晓坤, 王玉湾, 代月, 等. 贯叶连翘愈伤组织和不定根培养的研究[J]. 延边大学农学学报, 2012, 34（4）：282-288.
[30] Walker TS, Bais HP, Vivanco JM. Jasmonic acid-induced hypericin production in cell suspension cultures of Hyperieum perforatum L. （St. John’s wort）[J]. Phytochemistry, 2002, 60（3）：289-293.
[31] Sirvent T, Gibson D. Induction of hypericins and hyperforin in Hypericum perforatum L. in response to biotic and chemical elicitors[J]. Physiological and Molecular Plant Pathology, 2002, 60（6）：311-320.
[32] Bruno T, Antonio R, Giorgio P, et al. Induction of hypericins in Hypericum perforatum in response to chromium[J]. Fitoterapia, 2006, 77：164-170.
[33] 徐茂军, 董菊芳, 张刚. NO对金丝桃悬浮细胞生长及金丝桃素生物合成的促进作用研究[J]. 生物工程学报, 2005, 21（1）：66-70.

Related Articles 15

[1] TAO Na, LI Mao-xing, GUO Hua-chun. Optimization of Sweet Potato Genetic Transformation System Mediated by Agrobacterium rhizogenes [J]. Biotechnology Bulletin, 2023, 39(10): 175-183.

[2] LEI Chun-xia, LI Can-hui, CHEN Yong-kun, GONG Ming. Physiological and Biochemical Basis and Molecular Mechanism of Solanum tuberosum Tuberization [J]. Biotechnology Bulletin, 2022, 38(4): 44-57.

[3] ZHAO Zhong-ying, LI Qing-miao, TIAN Meng-liang, YANG Xiao-qian, KANG Yao, QIU Yu-jie, ZHANG Qing-ling, LIU Fan. Effects of ⁶⁰Co-γ Radiation on the Seedling Rate and Plant Characteristics of Pinellia ternata Callus [J]. Biotechnology Bulletin, 2021, 37(9): 142-151.

[4] DUAN Xu-guo, ZHANG Yu-hua, HUANG Ting-ting, DING Qian, LUAN Shu-yue, ZHU Qiu-yu. Synergetic Enhancing the Soluble Expression of Thermotoga maritima α-Glucan Phosphorylase by Chemical Chaperones and Induction Condition Optimization [J]. Biotechnology Bulletin, 2021, 37(8): 233-242.

[5] WANG Jian-yong, ZOU Yong-mei, GE Yan-bin, WANG Kai, XI Meng-li. Advance on Epigenetic Modification During Plant Callus Induction [J]. Biotechnology Bulletin, 2021, 37(8): 253-262.

[6] WANG Hai-bo, GUO Jun-yun. Molecular Cloning of Cold-induced JcDnaJ20p Promoter from Jatropha curcas and Its Functional Identification in Transgenic Tobacco [J]. Biotechnology Bulletin, 2021, 37(2): 24-31.

[7] LI Xiao-pei, WANG Si-ning, SHI Jing-jing, GAO Zhi-min. Progress of Plant Cuticular Wax Synthesis and Its Regulatory Factor WIN/SHN [J]. Biotechnology Bulletin, 2020, 36(12): 129-136.

[8] WU Li-fang, WEI Xiao-mei, LU Wei-dong. Embryonic Callus Induction of Sophora davidii and Their Somatic Embryogenesis and Germination [J]. Biotechnology Bulletin, 2019, 35(4): 13-19.

[9] FANG Luo, WU Xiao-qin. Suspension Culture of Somatic Cells of Pinus elliottii Against Brown Spot Needle Blight of Pine [J]. Biotechnology Bulletin, 2019, 35(3): 13-18.

[10] WANG Jun-yi, DONG Jin-jin, LIU Wei, CAO Fu-liang, WANG Gui-bin, WANG Yi-qiang. Research on the Growth,Browning and Flavonoid Accumulation of Ginkgo biloba Callus [J]. Biotechnology Bulletin, 2019, 35(2): 16-22.

[11] SONG Shao-zheng, YU Kang-ying, LU Rui, ZHANG Ting, CHEN Chao-jun, PAN Sheng-qiang, CHENG Yong, ZHOU Ming-ming. Deletion of the Marker Gene in Transgenic Goat Mammary Epithelial Cells by Cre/Loxp [J]. Biotechnology Bulletin, 2019, 35(12): 105-111.

[12] WANG Ling, LI Yan, DAI Wei-na, YAN Jing, ZHANG Chao-hong. Establishment and Optimization of Cell Suspension Culture System for Vitis vinifera [J]. Biotechnology Bulletin, 2018, 34(8): 80-86.

[13] MAO Pei-qi, LI Hou-hua, LI Ai, CAO Zhi-xiu, HAN Mei-ling, ZHANG Yan-long. Effects of Silver Nitrate on the Synthesis of Phenolic Compounds and the Expression Levels of Related Genes in Callus Browning Process of Paeonia ostii ‘Fengdan’ [J]. Biotechnology Bulletin, 2018, 34(8): 101-107.

[14] ZHANG Zheng-xue, LAN Zeng-quan, WU Tian. Establishment of Cell Suspension Culture System Based on the Leaf Callus in Noni [J]. Biotechnology Bulletin, 2018, 34(5): 142-147.

[15] CHEN Wan-qi, ZHANG Hong, WANG Jin-feng, CHEN Jun-jie, LIN Xiao-wen, LUO Jie-hua, XU Yuan, CHEN Yan-fang, LU Xing-yan. Optimal Cultivation of Rabdosia rubescens Hairy Roots and the Inhibition Test of Its Extract on Tumor Cell Growth [J]. Biotechnology Bulletin, 2018, 34(10): 122-128.

Recommended Articles

Metrics

Viewed

Full text

Abstract

Comments

Abstract

References

Related Articles

Recommended Articles

Metrics

Comments

TOP

Determination the Content of Total Flavonoids and Hypericin in Callus from Hypericum attenuatum Choisy

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	TAO Na, LI Mao-xing, GUO Hua-chun. Optimization of Sweet Potato Genetic Transformation System Mediated by Agrobacterium rhizogenes [J]. Biotechnology Bulletin, 2023, 39(10): 175-183.
[2]	LEI Chun-xia, LI Can-hui, CHEN Yong-kun, GONG Ming. Physiological and Biochemical Basis and Molecular Mechanism of Solanum tuberosum Tuberization [J]. Biotechnology Bulletin, 2022, 38(4): 44-57.
[3]	ZHAO Zhong-ying, LI Qing-miao, TIAN Meng-liang, YANG Xiao-qian, KANG Yao, QIU Yu-jie, ZHANG Qing-ling, LIU Fan. Effects of ⁶⁰Co-γ Radiation on the Seedling Rate and Plant Characteristics of Pinellia ternata Callus [J]. Biotechnology Bulletin, 2021, 37(9): 142-151.
[4]	DUAN Xu-guo, ZHANG Yu-hua, HUANG Ting-ting, DING Qian, LUAN Shu-yue, ZHU Qiu-yu. Synergetic Enhancing the Soluble Expression of Thermotoga maritima α-Glucan Phosphorylase by Chemical Chaperones and Induction Condition Optimization [J]. Biotechnology Bulletin, 2021, 37(8): 233-242.
[5]	WANG Jian-yong, ZOU Yong-mei, GE Yan-bin, WANG Kai, XI Meng-li. Advance on Epigenetic Modification During Plant Callus Induction [J]. Biotechnology Bulletin, 2021, 37(8): 253-262.
[6]	WANG Hai-bo, GUO Jun-yun. Molecular Cloning of Cold-induced JcDnaJ20p Promoter from Jatropha curcas and Its Functional Identification in Transgenic Tobacco [J]. Biotechnology Bulletin, 2021, 37(2): 24-31.
[7]	LI Xiao-pei, WANG Si-ning, SHI Jing-jing, GAO Zhi-min. Progress of Plant Cuticular Wax Synthesis and Its Regulatory Factor WIN/SHN [J]. Biotechnology Bulletin, 2020, 36(12): 129-136.
[8]	WU Li-fang, WEI Xiao-mei, LU Wei-dong. Embryonic Callus Induction of Sophora davidii and Their Somatic Embryogenesis and Germination [J]. Biotechnology Bulletin, 2019, 35(4): 13-19.
[9]	FANG Luo, WU Xiao-qin. Suspension Culture of Somatic Cells of Pinus elliottii Against Brown Spot Needle Blight of Pine [J]. Biotechnology Bulletin, 2019, 35(3): 13-18.
[10]	WANG Jun-yi, DONG Jin-jin, LIU Wei, CAO Fu-liang, WANG Gui-bin, WANG Yi-qiang. Research on the Growth,Browning and Flavonoid Accumulation of Ginkgo biloba Callus [J]. Biotechnology Bulletin, 2019, 35(2): 16-22.
[11]	SONG Shao-zheng, YU Kang-ying, LU Rui, ZHANG Ting, CHEN Chao-jun, PAN Sheng-qiang, CHENG Yong, ZHOU Ming-ming. Deletion of the Marker Gene in Transgenic Goat Mammary Epithelial Cells by Cre/Loxp [J]. Biotechnology Bulletin, 2019, 35(12): 105-111.
[12]	WANG Ling, LI Yan, DAI Wei-na, YAN Jing, ZHANG Chao-hong. Establishment and Optimization of Cell Suspension Culture System for Vitis vinifera [J]. Biotechnology Bulletin, 2018, 34(8): 80-86.
[13]	MAO Pei-qi, LI Hou-hua, LI Ai, CAO Zhi-xiu, HAN Mei-ling, ZHANG Yan-long. Effects of Silver Nitrate on the Synthesis of Phenolic Compounds and the Expression Levels of Related Genes in Callus Browning Process of Paeonia ostii ‘Fengdan’ [J]. Biotechnology Bulletin, 2018, 34(8): 101-107.
[14]	ZHANG Zheng-xue, LAN Zeng-quan, WU Tian. Establishment of Cell Suspension Culture System Based on the Leaf Callus in Noni [J]. Biotechnology Bulletin, 2018, 34(5): 142-147.
[15]	CHEN Wan-qi, ZHANG Hong, WANG Jin-feng, CHEN Jun-jie, LIN Xiao-wen, LUO Jie-hua, XU Yuan, CHEN Yan-fang, LU Xing-yan. Optimal Cultivation of Rabdosia rubescens Hairy Roots and the Inhibition Test of Its Extract on Tumor Cell Growth [J]. Biotechnology Bulletin, 2018, 34(10): 122-128.