Isolation and Screening of Plant Growth-promoting Rhizobacteria in Pepper and Their Disease-resistant Growth-promoting Characteristics

doi:10.13560/j.cnki.biotech.bull.1985.2019-0840

Abstract

Abstract: In order to obtain plant growth-promoting rhizobacteria（PGPR）of pepper and to explore their disease-resistant and growth-promoting characteristics,the PGPR were isolated and screened from pepper rhizosphere soil in Xuzhou city of Jiangsu province,using nitrogen fixation,inorganic phosphorus and organic phosphorus media. The strains were identified by morphological characteristics and 16SrDNA. Their abilities of fixing nitrogen,releasing phosphorus,secreting IAA and resistances to 4 pepper pathogenic diseases were investigated. The results showed that 13 PGPR strains of pepper were obtained and identified as Bacillus,Pseudomonas,Lelliottia,Siccibacter,Achromobacter,Microbacterium and Paenibacillus. All of them had function of fixing nitrogen,7 of them dissolved organic phosphorus,belonging to Lelliottia,Bacillus,Siccibacter,Microbacterium and Paenibacillus;5 dissolved solubilized inorganic phosphorus,belonging to Lelliottia,Bacillus,Siccibacter,and Pseudomonas;3 presented the ability of secreting IAA,belonging to Lelliottia,Siccibacter,Bacillus;and 5 demonstrated the resistances to diseases,belonging to Bacillus,Lelliottia,and Siccibacter. Pepper rhizosphere soil contains multi-functional PGPR,which has potential application value in agricultural production.

Key words: pepper, plant growth-promoting rhizobacteria, disease-resistant

YANG Mo, GAO Ting, LI Yan-jing, WEI Chong-yao, GAO Miao, MA Lian-ju. Isolation and Screening of Plant Growth-promoting Rhizobacteria in Pepper and Their Disease-resistant Growth-promoting Characteristics[J]. Biotechnology Bulletin, 2020, 36(5): 104-109.

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