Identification of Inorganic Phosphate-solubilizing Bacterium Mp1-Ha4 in Poplar Rhizosphere and Its Phosphate-solubilizing Mechanism

doi:10.13560/j.cnki.biotech.bull.1985.2019-0952

Abstract

Abstract: Inorganic phosphate-solubilizing bacteria can dissolve insoluble phosphate in soil,increase available phosphorus content in soil and thus promote plant growth. An inorganic phosphate-solubilizing bacterium Mp1-Ha4 screened from poplar rhizosphere soil was used as the research object. The strain was identified by molecular biology method,and its phosphate-solubilizing ability to calcium phosphate,aluminum phosphate and iron phosphate was determined. The dissolution kinetics of calcium phosphate by this strain within 9 days was also studied. The results showed that the inorganic phosphate-solubilizing bacterium Mp1-Ha4 was a Cedecea sp.,and its ability to dissolve calcium phosphate was much stronger than that to dissolve aluminum phosphate and iron phosphate. In NBRIP liquid culture medium,the strain’s dissolving ability to calcium phosphate reached 497.4 mg/L. During the phosphate solubilization process,the pH and titratable acidity of the culture medium showed significant negative and positive correlations with the phosphate solubilization amount,respectively. High performance liquid chromatography analysis showed that the strain secreted a large amount of organic acids,mainly including α-ketoglutaric acid,tartaric acid and malic acid,in the process of phosphorus removal. Secreting organic acids and reducing environmental pH may be the main mechanism of dissolving insoluble phosphate by inorganic phosphate-solubilizing bacterium Mp1-Ha4. Meanwhile,the efficient dissolution of calcium phosphate by the strain has great research and application prospects.

Key words: phosphate-solubilizing bacteria, calcium phosphate, organic acid

HAN Xue-jiao, ZENG Qing-wei, ZHAO Yu-ping. Identification of Inorganic Phosphate-solubilizing Bacterium Mp1-Ha4 in Poplar Rhizosphere and Its Phosphate-solubilizing Mechanism[J]. Biotechnology Bulletin, 2020, 36(3): 141-147.

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