Preliminary Study on Interaction Between Arabidopsis thaliana EBP1 Protein and RNA

doi:10.13560/j.cnki.biotech.bull.1985.2019-1066

Abstract

Abstract: Gene expression regulation is a hot spot in modern molecular biology,and the post-transcriptional regulation of RNA has attracted great concerns of researchers with the development of technology. RNA binding proteins（RBPs）,as an important post-transcriptional regulator,are involved in RNA alternative splicing,RNA stability,translation,and so on. ErbB3 binding protein（EBP1）is an RBP with highly conserved structure and functions,and is involved in the regulation of ribosome biogenesis and protein translation through binding a variety of RNAs such as rRNA,tRNA,and mRNA. However,few of RNA targets of EBP1 have been documented and the mechanism of interaction with its RNA target is still unknown. Here,the conserved RNA-binding domain in Arabidopsis EBP1 was predicted through bioinformatics analysis. “GUCUCUCACUGCGACGGCUU” sequence was directly bound by EBP1 in vitro. Further,mRNAs of three genes（AT1G24792,AT3G25211 and AT3G24320）were identified in RNA immunoprecipitation assay. Polysome profiling and cordycepin treatment experiments demonstrated that EBP1 significantly regulated the stability of these target mRNAs and their translation rates. Our results confirm that Arabidopsis EBP1 has the function of binding RNA,and regulates the post-transcriptional events of target RNAs,which provides useful data for further studying the biological mechanism of EBP1 post-transcriptional regulation.

Key words: EBP1, RNA binding protein, mRNA stability and translation

LI Xiao-yan, LI Chi-yu, YU Feng, LIAO Hong-dong. Preliminary Study on Interaction Between Arabidopsis thaliana EBP1 Protein and RNA[J]. Biotechnology Bulletin, 2020, 36(6): 35-45.

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