Overexpression of Grape ERECTA Enhances Growth and Thermo-tolerance of Arabidopsis

doi:10.13560/j.cnki.biotech.bull.1985.2019-1170

Abstract

Abstract: ERECTA gene expression in grape is induced by high temperature,and its expression level is high in young leaves and flowers. In order to study the role of grape ERECTA gene in plant growth/development and the response to high temperature,this gene was overexpressed in Arabidopsis,and its phenotype and thermo-tolerance were observed. The results showed that in the ERECTA-overexpressed Arabidopsis,the length,width and number of leaves increased,the pedicel became elongated,and pod length and plant height also increased significantly. Arabidopsis seeds were cultured at room temperature for 2 d after heat treatment. It was found that most Landsberg erecta（Ler）seedlings did not develop normally,while overexpressed seedlings ER-OE were not affected by heat stress. After Arabidopsis plants were treated at 40℃ for 4 d,ER-OE plants were subjected to little heat damage. The electrolyte leakage of leaves was Ler>Col>ER-OE. After recovery at 22℃for 5 d,the survival rate of Col,Ler and ER-OE was 33%,12% and 83%,respectively. ERECTA can not only improve the heat resistance of plants,but also increase the growth amount,which provides a new genetic resource for breeding new resistant varieties of grape,and has a promising application prospect in agricultural production.

Key words: grape, ERECTA, growth and development, high temperature, thermo-tolerance

LIU Min, FANG Yu-lin. Overexpression of Grape ERECTA Enhances Growth and Thermo-tolerance of Arabidopsis[J]. Biotechnology Bulletin, 2020, 36(6): 46-53.

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