Cloning and Functional Analysis of a Wheat Pathogenesis-Related Gene TaSec14

doi:10.13560/j.cnki.biotech.bull.1985.2019-1043

Abstract

Abstract: The Sec14 protein family in plants is involved in essential life processes such as phosphate metabolism and signal transduction. A phosphatidylinositol transporter gene was cloned from the powdery mildew-susceptible wheat cultivar Jing411. The length of open reading frame is 1 212 bp,encoding 403 amino acids. It contains a conserved SEC14 domain and has 80.45% homology with the phosphatidylinositol transporter PITP3（XP_010236107.1）in Brachypodium distachyon,designated as TaSec14. After inoculation with Blumeria graminis f. sp. tritici（Bgt）isolate,the expression of TaSec14 in Jing411 and its near isogenic line BJ-1 was consistent and higher than that in Brock. After knocking down the TaSec14 gene in Jing411 by virus-mediated gene silencing（VIGS）,the successful infection rate of the Bgt conidiaspores in the BSMV∶TaSec14 experimental group was lower than that in the GKP Buffer control group,and the deformity rate was higher than that of the control group. The above results indicated that reducing the expression of TaSec14 gene to some extent increased the resistance of susceptible wheat Jing411 to powdery mildew. It is speculated that TaSec14 gene may play a regulatory role in the interaction between wheat and powdery mildew.

Key words: wheat, TaSec14 gene, powdery mildew, VIGS

NIU Huan, FENG Jing-yun, HUANG Jian-guo, ZHANG Chao-qun, ZHANG Lu-lu, LIU Xiao-ying, WANG Zhen-ying. Cloning and Functional Analysis of a Wheat Pathogenesis-Related Gene TaSec14[J]. Biotechnology Bulletin, 2020, 36(6): 54-62.

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