Research Progress on CRISPR-Cas13-mediated RNA Editing System

doi:10.13560/j.cnki.biotech.bull.1985.2019-1204

Abstract

Abstract:

The immune defense system CRISPR-Cas,which is universal in bacteria and archaea,has now been extensively used in the field of biotechnology,especially CRISPR-Cas9 for targeting DNA. However,the RNA-targeted CRISPR-Cas technology is still in the preliminary application stage. The discovery of VI-type CRISPR-Cas system（CRISPR-Cas13）reveals RNA-guided RNA targeting. CRISPR-Cas13 is the system in the CRISPR-Cas family that targets only ssRNA so far,which lays a foundation for RNA targeting and RNA editing. It has recently been demonstrated that VI-type CRISPR-Cas system can be divided into 4 subtypes（A-D）based on Cas13 phylogeny. Here we review the classification and defense mechanisms of the latest RNA-targeting CRISPR-Cas13 systems,describes the advances in applications of CRISPR-Cas13 systems and in CRISPR-Cas13-mediated RNA editing system,and finally we analyze the remaining questions and highlight the prospects in CRISPR-Cas13-mediated RNA editing system.

Key words: CRISPR-Cas13, RNA editing, RNA technology, A>I, C>U

CHEN Min-jie, TANG Gui-yue, HONG Xiang-na, HAO Pei, JIANG Jing, LI Xuan. Research Progress on CRISPR-Cas13-mediated RNA Editing System[J]. Biotechnology Bulletin, 2020, 36(3): 1-8.

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