Role of Gut Microbiota in Stress-induced Hypertension in Rats

doi:10.13560/j.cnki.biotech.bull.1985.2019-1215

Abstract

Abstract: This work is to observe the variation of gut microbiota in stress-induced hypertension in rats and to investigate the role of gut microbiota in the pathogenesis of stress-induced hypertension in rats. The model of stress-induced hypertension rats was prepared by：Rats were subjected to plantar electrical stimulation and noise twice a day for 2 h at an interval of 4 h for consecutive 14 d. Measurement of blood pressure was conducted by：arterial catheterization in rats,and then blood pressure was recorded by Powerlab system. The model of pseudo germ-free rats was prepared by adding 4 antibiotics of 1 g/L ampicillin,500 mg/L vancomycin,1 g/L neomycin,1 g/L metronidazole file in their drinking water for 4 weeks. The 16S rRNA V3-V4 region sequence of microbe in rat feces was determined by Illumina MiSeq sequencing technique,and the community structure and diversity were compared and analyzed. Chronic stress increased blood pressure,heart rate and serum norepinephrine concentration in rats. α diversity analysis showed that the richness and diversity of gut microbiota decreased in stress rats,and β diversity analysis showed that the species composition of gut microbiota in stress group decreased and the difference became greater. In the pseudo germ-free rats,the gut microbiota species was simple with only a small number of Proteobacteria and the chronic stress did not increase blood pressure,heart rate as well as serum norepinephrine concentration. Stress may cause disorders of gut microbiota in rats and stress-induced hypertension may depend on gut microbiota.

Key words: stress, hypertension, gut microbiota, rat

WU Qin, XU Zi-yang, LIU Li-ping, ZHANG Wen-ying, SONG Si-yuan. Role of Gut Microbiota in Stress-induced Hypertension in Rats[J]. Biotechnology Bulletin, 2020, 36(2): 83-90.

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