Fluorescent Detection of Ag+ Using Graphene and Functional Nucleic Acid

doi:10.13560/j.cnki.biotech.bull.1985.2019-1248

Abstract

Abstract: Based on the principle of mismatched binding Ag⁺ of nucleic acid aptamer,using the different binding forces of graphene to single-stranded DNA and double-stranded DNA,a new fluorescent sensor with C-Ag⁺-C and graphene binding was designed and developed. A high sensitivity,fast and convenient fluorescence detection method was developed to achieve the Ag⁺ detection in turn on form. Under the optimization of detection conditions,the turn on fluorescent aptasensor offered a linear detection range of 150-400 nmol/L with a detection limit of 23 nmol/L for Ag⁺. The recovery rate of the method was 96.6% for Ag⁺-labeled detection in the experimental samples of drinking water,showing the ability to be used for actual sample analysis.

Key words: Ag⁺, base mismatch, graphene, fluorescence detection

WU Xue-qi, ZHANG Li-pei, CAO Yang, WEN Xiao-gang, ZHOU Xiao-hong. Fluorescent Detection of Ag⁺ Using Graphene and Functional Nucleic Acid[J]. Biotechnology Bulletin, 2020, 36(8): 210-216.

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Fluorescent Detection of Ag⁺ Using Graphene and Functional Nucleic Acid

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