Isolation of an Acetoin-producing Strain Resistant to High Concentration Glucose and Identification of Metabolites

doi:10.13560/j.cnki.biotech.bull.1985.2020-0119

Abstract

Abstract: In order to obtain an acetoin-producing strain that is resistant to high concentrations of glucose,a new strain was screened from tobacco field soil using high-sugar medium and acetoin coloring solution;then the screened strain was sequenced and identified by whole genome technology and 16S rDNA sequence alignment analysis,and tolerance of the strain was tested at different concentrations of glucose in the medium. Finally,the GC-MS（gas chromatography-mass spectrometry）was used to qualitatively analyze the fermentation products of the strain and the capacity of acetoin-producing was analyzed by preliminary fermentation for 72 h. The results showed that the strain obtained was Staphylococcus aureus and named as PX03. In the glucose tolerance test,the strain PX03 still grew in a medium containing 500 g/L glucose,with an OD₆₀₀ of 65;grew the best at a concentration of 300 g/L glucose,with an OD₆₀₀ of 100. GC-MS qualitative analysis revealed that the strain produced a large amount of aroma components,including acetoin（22.51%）,2,3-butanediol（21.29%）,acetic acid（13.97%）,3,4-dihydroxy-3,4-dimethyl-2,5-hexanedione（8.52%）,pyranone（5.24%）and 3-methyl-bbutanoic acid（4.47%）. The preliminary fermentation results demonstrated that the yield of acetoin of this strain reached 41 g/L.

Key words: microbial fermentation, Staphylococcus, acetoin, high glucose tolerance

HUO Ming-yue, ZHANG Ge, SU Yu-long, LI Xing, ZHANG Hai-bo, ZHENG Rong, LIU Hao-bao. Isolation of an Acetoin-producing Strain Resistant to High Concentration Glucose and Identification of Metabolites[J]. Biotechnology Bulletin, 2020, 36(8): 53-60.

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