The Mechanism of Dissolving Inorganic Phosphorus by Bacillus megaterium ZS-3 and Its Growth Promotion of Cinnamomum camphora

doi:10.13560/j.cnki.biotech.bull.1985.2020-0114

Abstract

Abstract: The purpose of this study is to explore the inorganic phosphorus-dissolving characteristics and corresponding phosphorus-dissolving mechanism of Bacillus megaterium ZS-3,and to provide a theoretical basis and technical approach for utilizing and improving the utilization rate of poorly soluble phosphorus resources in soil. NBRIP medium,molybdenum anti-colorimetric method,dilution coating method,and acid-base titration method were applied to determine the amount of phosphorus solubilized,colony growth and titratable acidity of the strain. Scanning electron microscope（SEM）was used to observe the surface morphology of calcium phosphate Ca₃（PO₄）₂. High performance liquid chromatography and p-nitrophenyl phosphate disodium were adapted to measure the types of organic acids,content and activity of phosphatase in the fermentation broth. Selecting 2 different types of soil for pot experiments,the effects of strain ZS-3 on the growth of Cinnamomum camphora and the physicochemical properties of soil were checked. As results,the maximum amount of dissolved phosphorus by strain ZS-3 occurred at 96 h with 188 μg/mL when it was continuously cultured for 168 h. The maximum number of cells reached 2.34×10⁹ CFU/mL at 72 h. The pH dropped the most quickly at early 24 h from initial 8.06 to 5.14. The titratable acidity was slowly increasing. SEM experiment showed that the strain ZS-3 caused the calcium phosphate surface uneven and rough after interacting with it. The activities of acid phosphatase and alkaline phosphatase were low during the dissolution by ZS-3. A large amount of organic acids were generated during the ZS-3 culturing,mainly acetic acid and formic acid,their contents were 484.12 ng/μL and 255.1 ng/μL,respectively. The application of strain ZS-3 promoted the growth of C. camphorain planted in two different types of soil. The soil available phosphorus content increased and there was more significant increase of available phosphorus content in low phosphorus soil. In sum,the strain ZS-3 can efficiently dissolve inorganic phosphorus and promote the growth of C. camphora,and is expected to provide resources for the development of phosphorus-solubilizing microbial fertilizer.

Key words: Bacillus megaterium, phosphorus-dissolving microorganism, organic acid, growth-promoting effect

QIAN Ting, YE Jian-ren. The Mechanism of Dissolving Inorganic Phosphorus by Bacillus megaterium ZS-3 and Its Growth Promotion of Cinnamomum camphora[J]. Biotechnology Bulletin, 2020, 36(8): 45-52.

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