Isolation and Identification of Lignin-degrading Bacteria from the Gut of Termite and Their Degradation Characteristics

doi:10.13560/j.cnki.biotech.bull.1985.2020-0128

Abstract

Abstract: The objectives of this work are to enrich and isolate lignin-degrading bacteria from the gut of termite,and to study their degradation characteristics. A lignin-degrading bacterium was first isolated from the gut of a termite species,Microtermes pakistanicus,and then identified by 16S rRNA gene sequence analysis for their phylogeny property. Further,with a variety of evaluation methods,such as FTIR and TGA,the degradation characteristics of lignin for the strain were analyzed. As results,a strain MP-132 was identified as Raoultella ornithinolytica. The degradation efficiency was up to 53.2% after 7 d culture when alkaline lignin was used as sole carbon source. Laccase and lignin peroxidase peroxidase reached maximum activities 32 U/L and 105.6 U/L,respectively. Moreover,strain MP-13 led the azure B and aniline blue decolorized. The results from the FTIR and TGA analysis showed that strain MP-132 uniquely presented an attractive capability to deconstruct lignin components. In conclusion,this work systematically reveals the degradation characteristics of strain MP-132,which may provide fine germplasm resources for the biodegradation of lignin.

Key words: lignin, Raoultella, termite, degradation

LI Feng, HUANG Shu-shi. Isolation and Identification of Lignin-degrading Bacteria from the Gut of Termite and Their Degradation Characteristics[J]. Biotechnology Bulletin, 2020, 36(8): 61-68.

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