Screening and Degradation Effect of Lignin-degrading Bacteria in Termite Nurseries

doi:10.13560/j.cnki.biotech.bull.1985.2021-0626

Abstract

Abstract:

In order to select strains that can effectively degrade lignin,waste termite nests were used as a source for screening and re-screening was conducted with nitrogen-free medium,alkaline lignin medium,aniline blue medium and guaiacol medium. The screened strains were identified by 16S rRNA as well as morphological analysis. The alkaline lignin before and after degradation was observed in a characterized structure using scanning electron microscopy and Fourier infrared spectroscopy. The results showed that a strain of lignin-degrading bacteria was identified and named R. ornithinolytica RS-1. The lignin degradation rate reached a maximum of 24.21% in the first 3 d of fermentation with alkaline lignin as substrate,while the enzyme activities of 3 lignin-degrading enzymes（peroxidase,manganese peroxidase,and laccase）reached a maximum of 177.42,1466.67 and 6.94 U/L,respectively. Scanning electron microscopy and infrared spectroscopy revealed significant structural changes after treatment,indicating that the bacteria can be used as an effective strain for lignin degradation.

Key words: termite nurseries, alkaline lignin, lignin-degrading bacteria, raoulla ornithinolytica

HAN Dong-jing, WANG Zhi-hua, ZHOU Ning, LIU Guo-qing, YANG Shao-hua, WANG Wen-jun. Screening and Degradation Effect of Lignin-degrading Bacteria in Termite Nurseries[J]. Biotechnology Bulletin, 2022, 38(3): 113-120.

Figures/Tables 8

Fig. 1 Lignin concentration standard curve

Fig. 2 Growth of strain RS-1 on different media A：Nitrogen-free medium. B：Aniline blue medium. C：Guaiacol medium

Fig. 3 Scanning electron microscopic observation（A）and growth curve（B）of strain RS-1

Fig. 4 Phylogenetic tree of strain RS-1

Fig. 5 Variation of relevant enzyme activity over time during lignin degradation

Fig. 6 Lignin concentration and degradation rate over time

Fig. 7 Scanning electron micrographs before and after alkaline lignin treatment A：Untreated alkaline lignin. B：Alkaline lignin from bacterial RS-1 treatment for 7 d

Fig. 8 Variations in FIR spectra untreated and treated alkaline lignin treatment

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