Abstract:

Using iron oxide red paint and acrylate as coating base,4 different nanomaterials（0.1%,W/V）were added to make 2 types of antifouling coatings. The two types of coating performed similarly in the context of antifouling scores,but significantly more Ciona intestinalis attached on the iron oxide red paint coatings than acrylate ones. Biofilm samples were collected from marine hanging plates,the metagenome DNA samples from the 28^th day biofilm were subjected to high-throughput sequencing of full-length 16S rRNA,and the composition differences of prokaryotic flora between 2 coatings were analyzed. The results indicated that：1）the alpha diversity of prokaryotic flora in the acrylate coating was higher than that in the iron oxide red paint coating;2）interestingly,nanomaterials didn’t result in the significant composition changes in the prokaryotic flora on the both coatings;and 3）there were significant differences in the abundance of several bacterial strains between two types of coatings,especially for two dominant bacteria Neptuniibacter sp. CAR-SF and Cellvibrionales unclassified at 4% on the iron oxide red paint coating but almost zero on the acrylate coating. The observed OTU numbers of the two bacteria on 2 coatings were of over 100-fold difference,and the qPCR results confirmed that the contents of Neptuniibacter genus on 2 coatings differed remarkably. Whether the decrease in the amount of ascidian attached to the acrylate-based coating is related to the above bacterial content difference is worthy of further study.

Key words: biofilm, iron oxide red paint, acrylate, diversity of prokaryotic flora, ascidian