Short Tandem Target Mimic and Its Application in Analyzing Plant miRNA Functions

doi:10.13560/j.cnki.biotech.bull.1985.2020-0257

Abstract

Abstract:

MicroRNA（miRNA）,a class of endogenous non-coding RNAs in about 21-24 nucleotides length,regulates gene expression by affecting the stability of target mRNA or by regulating the process of translation. With the development of modern molecular biology technology,more and more studies demonstrated that miRNAs play an important role in the growth and development of plants. However,in the miRNA function research,more effective and widely applicable methods are needed. Here,a specific miRNA targeting method is introduced,namely Short Tandem Target Mimic（STTM）. The basic characteristics,function mechanism of STTM,comparison with other similar technologies,and its application in the study of miRNA function in plants are described,which may provide a technical reference for the study of plant miRNA function in the future.

Key words: plant, STTM, miRNA, regulatory mechanism

ZHENG Wen-qing, ZHANG Qian, DU Liang. Short Tandem Target Mimic and Its Application in Analyzing Plant miRNA Functions[J]. Biotechnology Bulletin, 2020, 36(12): 256-264.

Figures/Tables 4

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技术	结构	作用机制	优点	缺点
TM	1个结合位点无连接序列3个碱基的错配	RISC-RNA interaction	构建较容易	不适用于有多个成员的miRNA家族
SP	4-15个结合位点连接序列4 nt2个碱基的错配	RISC-RNA interaction	在动物中沉默效果较好	构建困难
STTM	2个结合位点连接序列48-88 nt3个碱基的错配	RISC-RNA interaction SDN-mediated degradation	构建较容易,效率高特异性强,表型稳定	不适用于低表达丰度的miRNA家族

技术	结构	作用机制	优点	缺点
TM	1个结合位点无连接序列3个碱基的错配	RISC-RNA interaction	构建较容易	不适用于有多个成员的miRNA家族
SP	4-15个结合位点连接序列4 nt2个碱基的错配	RISC-RNA interaction	在动物中沉默效果较好	构建困难
STTM	2个结合位点连接序列48-88 nt3个碱基的错配	RISC-RNA interaction SDN-mediated degradation	构建较容易,效率高特异性强,表型稳定	不适用于低表达丰度的miRNA家族