Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (12): 256-264.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0257

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Short Tandem Target Mimic and Its Application in Analyzing Plant miRNA Functions

ZHENG Wen-qing(), ZHANG Qian, DU Liang()   

  1. College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083
  • Received:2020-03-10 Online:2020-12-26 Published:2020-12-22
  • Contact: DU Liang E-mail:zhengwenqing@bjfu.edu.cn;duliang@bjfu.edu.cn

Abstract:

MicroRNA(miRNA),a class of endogenous non-coding RNAs in about 21-24 nucleotides length,regulates gene expression by affecting the stability of target mRNA or by regulating the process of translation. With the development of modern molecular biology technology,more and more studies demonstrated that miRNAs play an important role in the growth and development of plants. However,in the miRNA function research,more effective and widely applicable methods are needed. Here,a specific miRNA targeting method is introduced,namely Short Tandem Target Mimic(STTM). The basic characteristics,function mechanism of STTM,comparison with other similar technologies,and its application in the study of miRNA function in plants are described,which may provide a technical reference for the study of plant miRNA function in the future.

Key words: plant, STTM, miRNA, regulatory mechanism