Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (12): 256-264.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0257
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ZHENG Wen-qing(), ZHANG Qian, DU Liang()
Received:
2020-03-10
Online:
2020-12-26
Published:
2020-12-22
Contact:
DU Liang
E-mail:zhengwenqing@bjfu.edu.cn;duliang@bjfu.edu.cn
ZHENG Wen-qing, ZHANG Qian, DU Liang. Short Tandem Target Mimic and Its Application in Analyzing Plant miRNA Functions[J]. Biotechnology Bulletin, 2020, 36(12): 256-264.
技术 | 结构 | 作用机制 | 优点 | 缺点 |
---|---|---|---|---|
TM | 1个结合位点无连接序列3个碱基的错配 | RISC-RNA interaction | 构建较容易 | 不适用于有多个成员的miRNA家族 |
SP | 4-15个结合位点连接序列4 nt2个碱基的错配 | RISC-RNA interaction | 在动物中沉默效果较好 | 构建困难 |
STTM | 2个结合位点连接序列48-88 nt3个碱基的错配 | RISC-RNA interaction SDN-mediated degradation | 构建较容易,效率高特异性强,表型稳定 | 不适用于低表达丰度的miRNA家族 |
技术 | 结构 | 作用机制 | 优点 | 缺点 |
---|---|---|---|---|
TM | 1个结合位点无连接序列3个碱基的错配 | RISC-RNA interaction | 构建较容易 | 不适用于有多个成员的miRNA家族 |
SP | 4-15个结合位点连接序列4 nt2个碱基的错配 | RISC-RNA interaction | 在动物中沉默效果较好 | 构建困难 |
STTM | 2个结合位点连接序列48-88 nt3个碱基的错配 | RISC-RNA interaction SDN-mediated degradation | 构建较容易,效率高特异性强,表型稳定 | 不适用于低表达丰度的miRNA家族 |
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