The Expression of LEPRb mRNA in the Estrus Cycle of Different Sheep Strains

doi:10.13560/j.cnki.biotech.bull.1985.2017.05.020

Abstract

Abstract: The aim of the study is to explore LEPR expression characteristics in seasonal estrus of sheep. Chinese merino（seasonal estrus sheep）and Duolang sheep（perennial estrus sheep）were selected to determine the expression changes of LEPRb mRNA in hypothalamus,ovaries and uterus in different stage of estrus cycle by quantitative real-time PCR. The results showed that during the whole estrus cycle,except the metaestrus,the LEPRb levels in the ovarian and uterus were found to be significantly lower than the levels in hypothalamus of Chinese Merino,but the results in metaestrus showed the reverse. Although the expression level of LEPRb varied in the same tissue of 2 cultivars during estrus stage,the trend was similar. Moreover,the LEPRb expression levels in different tissues of Chinese Merino were significantly higher than those in Duolang sheep. The results indicate that the expression variation of LEPRb is correlated with sheep estrus.

Key words: sheep, LEPRb, expression level, estrus cycle, real-time quantitative PCR

LU Shou-liang LI Liang-yuan GAO Lei SHEN Min WAN Peng-cheng SHI Guo-qing DAI Rong. The Expression of LEPRb mRNA in the Estrus Cycle of Different Sheep Strains[J]. Biotechnology Bulletin, 2017, 33(5): 139-144.

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