Research Progress on the Construction and Application of Pickering Emulsion Enzymatic Reaction System

doi:10.13560/j.cnki.biotech.bull.1985.2020-0792

Abstract

Abstract:

Pickering emulsion is stabilized by solid particles and possesses the advantages of anti-agglomeration,good stability,easy separation of particles,low cost and low toxicity. Studies have shown that its application in heterogeneous enzyme-catalyzed reactions may greatly increase the interface reaction area and accelerate the mass transfer rate. This article reviews the solid particles commonly used to stabilize Pickering emulsions and the effect of particle surface modification on emulsion stability. On this basis,the article further discusses the application of Pickering emulsion in the enzyme reaction system and the smart Pickering emulsion enzyme reaction system with “switch” regulation in detail,and prospects the development of Pickering emulsion enzyme catalytic reaction system. It aims to provide more ideas for constructing a more efficient and green enzyme-catalyzed reaction.

Key words: Pickering emulsion, solid particles, enzyme-catalyzed, immobilized enzyme, intelligent regulation

XU Liu-jia, ZHENG Ming-ming. Research Progress on the Construction and Application of Pickering Emulsion Enzymatic Reaction System[J]. Biotechnology Bulletin, 2021, 37(2): 187-194.

Figures/Tables 3

References 52

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颗粒种类	改性前		改性后
颗粒种类	直径/μm	三相接触角/°	直径/μm	三相接触角/°
淀粉球晶	3.97	39.5	3.88	74.4
大米淀粉	7.61	42.5	7.55	67.6
玉米淀粉	14.98	33.6	14.38	60.7
马铃薯淀粉	26.93	46.1	24.77	54.4

颗粒种类	改性前		改性后
颗粒种类	直径/μm	三相接触角/°	直径/μm	三相接触角/°
淀粉球晶	3.97	39.5	3.88	74.4
大米淀粉	7.61	42.5	7.55	67.6
玉米淀粉	14.98	33.6	14.38	60.7
马铃薯淀粉	26.93	46.1	24.77	54.4

酶的种类	稳定剂/载体	酶促反应	有机溶剂	转化率	参考文献
脂肪酶AYS	介孔碳球	植物甾醇和α-亚麻酸的酯化反应	-	1.5 h,92%	[22]
脂肪酶CALB	纳米聚合体PEG-b-P（S-co-TMI）	1-己醇与己酸的酯化反应	甲苯	24 h,80%	[38]
脂肪酶	海藻酸盐复合粒子E@Alg@s-TiO₂	1-己醇与己酸的酯化反应	正己烷	4 h,85-95%	[39]
脂肪酶CALB	纳米共聚物P（St-co-GMA）	己醇和己酸的酯化反应	庚烷	24 h,96.5%	[40]
脂肪酶CALB	二氧化硅纳米花	甲醇和废弃油生产生物柴油	环己烷	8.11 h,98.5%	[41]
脂肪酶CALB	功能化多壁碳纳米管MWCNTs-NH₂	马齿苋种子油和甲醇生产生物柴油	正庚烷	11.06 h,95.2%	[42]
脂肪酶CALB	介孔纳米二氧化硅	1-己醇与己酸的酯化反应	甲苯	20 min,88%	[43]

酶的种类	稳定剂/载体	酶促反应	有机溶剂	转化率	参考文献
脂肪酶AYS	介孔碳球	植物甾醇和α-亚麻酸的酯化反应	-	1.5 h,92%	[22]
脂肪酶CALB	纳米聚合体PEG-b-P（S-co-TMI）	1-己醇与己酸的酯化反应	甲苯	24 h,80%	[38]
脂肪酶	海藻酸盐复合粒子E@Alg@s-TiO₂	1-己醇与己酸的酯化反应	正己烷	4 h,85-95%	[39]
脂肪酶CALB	纳米共聚物P（St-co-GMA）	己醇和己酸的酯化反应	庚烷	24 h,96.5%	[40]
脂肪酶CALB	二氧化硅纳米花	甲醇和废弃油生产生物柴油	环己烷	8.11 h,98.5%	[41]
脂肪酶CALB	功能化多壁碳纳米管MWCNTs-NH₂	马齿苋种子油和甲醇生产生物柴油	正庚烷	11.06 h,95.2%	[42]
脂肪酶CALB	介孔纳米二氧化硅	1-己醇与己酸的酯化反应	甲苯	20 min,88%	[43]