Advances in Analysis Methods of Mass Spectrometry-based Metabolomics

doi:10.13560/j.cnki.biotech.bull.1985.2020-1383

Abstract

Abstract:

Mass spectrometry（MS）-based metabolomics analyses are now widely applied to identify and quantify small-molecular metabolites in biological samples for uncovering endogenous alterations in cells after their exposure to exogenous stimulus. Duo to the complicated components in biological samples and a big span of polarities of metabolites ranged from hydrophilic to hydrophobic metabolites,a series of extraction protocols and the matched liquid chromatography（LC）and MS conditions are required to obtain the quantitative and qualitative information of target components. To some extent,the diversity of structure and physicochemical properties of small molecule metabolites limits the high-throughput acquirement of whole metabolic profiles from biological samples. Present review focuses on the introduction and discussion of extraction methods of varied metabolites,as well as their corresponding LC-MS conditions.

Key words: mass spectrometry, metabolomics, lipidomics

TIAN He, SHUI Guang-hou. Advances in Analysis Methods of Mass Spectrometry-based Metabolomics[J]. Biotechnology Bulletin, 2021, 37(1): 24-32.

Figures/Tables 2

References 37

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	¹H-NMR	GC-MS	LC-MS
灵敏度	nmol/L	amol/L	amol/L
特异性	质子信号易重叠干扰定性、定量	强	强
稳定性	无需内标校准	依赖内标校准	依赖内标校准
样品前处理	要求低或无需前处理	要求高,除蛋白与杂质	要求高,除蛋白与杂质
代谢组定性数目	一般小于100个	几千到几万个	几千到几万个
检测代谢物种类	氨基酸、有机酸、碳水化合物等	热稳定、易挥发或衍生化后易挥发	热不稳定、难挥发
分子量	动物样品一般小于400,植物样品通常小于800	小于1 000	分子量没有上限

	¹H-NMR	GC-MS	LC-MS
灵敏度	nmol/L	amol/L	amol/L
特异性	质子信号易重叠干扰定性、定量	强	强
稳定性	无需内标校准	依赖内标校准	依赖内标校准
样品前处理	要求低或无需前处理	要求高,除蛋白与杂质	要求高,除蛋白与杂质
代谢组定性数目	一般小于100个	几千到几万个	几千到几万个
检测代谢物种类	氨基酸、有机酸、碳水化合物等	热稳定、易挥发或衍生化后易挥发	热不稳定、难挥发
分子量	动物样品一般小于400,植物样品通常小于800	小于1 000	分子量没有上限