Study on the Algicidal Characteristics and Physiological Response of Microbacterium sp. CBA01 to Phaeocystis globosa

doi:10.13560/j.cnki.biotech.bull.1985.2021-0161

Abstract

Abstract:

The harmful algae blooms have seriously affected marine ecological environment and mariculture industry,and the exploitation of algae-lying bacteria is one of the effective biological methods to control the harmful algae blooms. This work aims to investigate the algicidal characteristics and preliminary mechanism of a strain of Microbacterium sp. CBA01,one of the 4 strains of epiphytic bacterium isolated from Cyanobacterium sp. SCSIO-45682,with strong algicidal effect on Phaeocystis globosa. The co-culture mode of bacterial fermentation broth and P. globosa was used to determine the algae-lying effect of the strain CBA01,and the algicidal characteristics were studied by removing bacteria,different temperature and different pH,respectively. Furthermore,algal cells about the response rules of malondialdehyde（MDA）and antioxidant related enzyme system were determined. The strain CBA01 fermentation broth and sterile supernatant had obvious algicidal effect on P. globosa,but the mycelia itself showed no algicidal activity. The algicidal effect enhanced with the increase of fermentation time and the addition amount of fermentation broth,and the decrease of the initial concentration of algae. After adding the CBA01 sterile supernatant,the MDA content and the activities of superoxide dismutase（SOD）and peroxidase（POD）significantly increased in the early growth stage of P. globosa. In conclusion,the strain CBA01 caused the lysis of P. globosa by secreting extracellular algicidal substances. The active algicidal substances are of certain high temperature resistance,acid resistance but weak alkaline resistance. And the algicidal substances produced by the strain CBA01 may either have strong oxidation activity or induce the algal cells to produce excess reactive oxygen species（ROS）,which might be an important mechanism of alginolytic effect.

Key words: Microbacterium sp. CBA01, harmful algae blooms, Phaeocystis globosa, algicidal characteristics, physiological response

WANG Ling, XIANG Wen-zhou, WEI Hua-ning, LV Jin-ting, WU Hua-lian, WU Hou-bo. Study on the Algicidal Characteristics and Physiological Response of Microbacterium sp. CBA01 to Phaeocystis globosa[J]. Biotechnology Bulletin, 2021, 37(10): 91-99.

Figures/Tables 6

Fig. 1 Growth curve of symbiotic bacteria CAB01

Fig. 2 The effect of different fermentation time (A) and addition amount of bacterial solution (B) and different initial concentration of P. globosa (C) on algicidal activity

Fig. 3 The effect of different addition treatment of bacter-ial solution on algicidal activity

Fig. 4 Algicidal efficiency of CBA01 sterile filtrate trea-ted with different temperature (A) and pH (B) conditions on P. globosa

Fig. 5 Effect of sterile supernatant on the activity of antioxidant enzymes of P. globosa cells ** indicates a very significantly difference from the control group（P<0.01）, the same below

Fig 6 Effect of sterile supernatant on chlorophyll a acti-vity of P. globosa cells

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