Transcriptome Identification of Terpenoid Synthase Genes in Jasminum sambac and Their Expressions Responding to Exogenous Hormones

doi:10.13560/j.cnki.biotech.bull.1985.2021-0716

Abstract

Abstract:

The objective of this work is to explore the role of terpenoid synthase（TPS）in the synthesis of terpenoid in jasmine and its expression under exogenous hormones treatments,which will lay a theoretical basis for further study of the function of J. sambac TPS（JsTPS）family and its role in exogenous hormones stress. Based on the transcriptome data of jasmine in this study,bioinformatics was applied to identify and analyze the JsTPS family. Real-time fluorescence quantitative detection was to analyze the expressions of JsTPS family in different tissues and exogenous hormones treatments. The result showed that there were 8 TPS genes of having both Terpene_synthase and Terpene_synthase_C conserved domain in JsTPS family,named JsTPS1 - JsTPS8 respectively. The protein lengths of JsTPS genes ranged from 163 to 844 amino acids. The molecular weights of JsTPS genes ranged from 19 103.6 to 96 887.4 kD. And they were mainly located in cytoplasm and chloroplast. The JsTPS genes were divided into 4 subfamilies（a,b,e/f,and g）based on phylogenetic relationships. The analysis of qRT-PCR showed that the expressions of gene JsTPS1,JsTPS2,JsTPS3,JsTPS5,JsTPS6,and JsTPS7 were high in petals;also the expressions of the JsTPS1,JsTPS2,JsTPS3,JsTPS5,JsTPS6 and JsTPS7 were induced by IAA,GA,SA and MeJA. The MeJA significantly induced the expressions of 6 JsTPS genes,and the relative expression reached the maximum at 4-9 h. The induction from high to low was MeJA > IAA > GA > SA. J. sambac contained 8 TPS family members which were mainly expressed in petals and closely related to the formation of aroma in J. sambac. They were up-regulated under IAA and MeJA inductions. GA and SA treatments may inhibit the expressions of partial JsTPS genes.

Key words: Jasminum sambac, terpenoid synthase, bioinformatics, expression analysis

HONG Ya-ping, CHEN Xue-jin, WANG Peng-jie, GU Meng-ya, GAO Ting, YE Nai-xing. Transcriptome Identification of Terpenoid Synthase Genes in Jasminum sambac and Their Expressions Responding to Exogenous Hormones[J]. Biotechnology Bulletin, 2022, 38(3): 41-49.

Figures/Tables 7

References 31

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基因名称 Gene name	上游引物 Forward primer（5'-3'）	下游引物 Reverse primer（5'-3'）
JsTPS1	TTGAAGTTGCTCGACGCTCT	GCATCTCCAAGGCTGCACTA
JsTPS2	AGCACGATGACCAGACAGTG	GCACCAGTCGAGCGTAATCT
JsTPS3	GATGATCGGAGGTGGCGATT	TGCAAAATGTGACGCTTCGG
JsTPS4	GGTGGTGGGAGAATCTTGGG	CAAGGAATGAAGCGCTTCCG
JsTPS5	GAGAACCTCCACCTCACTGC	AAGCCATTTTCGCAAGCTCG
JsTPS6	TACCACAGGAGCATGGCAAG	AATCGGCAACTCCCTGGAAG
JsTPS7	GCTCTACCCGCAACTCTTGT	TCGTCTGCAGGATACCTTGC
JsTPS8	GCTTGAGCTCTTTGAGGCCT	GTGGTTCAAATGCGACTCCA

基因名称 Gene name	上游引物 Forward primer（5'-3'）	下游引物 Reverse primer（5'-3'）
JsTPS1	TTGAAGTTGCTCGACGCTCT	GCATCTCCAAGGCTGCACTA
JsTPS2	AGCACGATGACCAGACAGTG	GCACCAGTCGAGCGTAATCT
JsTPS3	GATGATCGGAGGTGGCGATT	TGCAAAATGTGACGCTTCGG
JsTPS4	GGTGGTGGGAGAATCTTGGG	CAAGGAATGAAGCGCTTCCG
JsTPS5	GAGAACCTCCACCTCACTGC	AAGCCATTTTCGCAAGCTCG
JsTPS6	TACCACAGGAGCATGGCAAG	AATCGGCAACTCCCTGGAAG
JsTPS7	GCTCTACCCGCAACTCTTGT	TCGTCTGCAGGATACCTTGC
JsTPS8	GCTTGAGCTCTTTGAGGCCT	GTGGTTCAAATGCGACTCCA

基因 Gene	转录组基因 Transcriptome gene	氨基酸数目 Number of amino acids/aa	分子量 MW/kD	等电点 pI	平均亲水性 GRAVY	亚细胞定位 Subcellular location	功能预测 Predicted functional
JsTPS1	c33849.graph_c0	552	64070.58	5.25	-0.191	叶绿体 Chloroplast	大根香叶烯D合酶 Germacrene D synthase
JsTPS2	c29553.graph_c0	593	69302.16	5.78	-0.371	细胞质 Cytoplasm	-
JsTPS3	c30319.graph_c0	600	69530.43	5.90	-0.351	叶绿体 Chloroplast	-
JsTPS4	c19904.graph_c0	584	66906.02	5.18	-0.326	叶绿体 Chloroplast	-
JsTPS5	c33314.graph_c0	540	62628.94	5.63	-0.245	细胞质 Cytoplasm	α-法尼烯合酶 α-farnesene synthase
JsTPS6	c32177.graph_c0	584	68311.93	5.55	-0.390	叶绿体 Chloroplast	-
JsTPS7	c27401.graph_c0	844	96887.40	6.09	-0.343	细胞质 Cytoplasm	（E,E）-香叶基芳樟醇合酶（E,E）-geranyllinalool synthase
JsTPS8	c44781.graph_c0	163	19103.06	5.64	-0.071	-	-

基因 Gene	转录组基因 Transcriptome gene	氨基酸数目 Number of amino acids/aa	分子量 MW/kD	等电点 pI	平均亲水性 GRAVY	亚细胞定位 Subcellular location	功能预测 Predicted functional
JsTPS1	c33849.graph_c0	552	64070.58	5.25	-0.191	叶绿体 Chloroplast	大根香叶烯D合酶 Germacrene D synthase
JsTPS2	c29553.graph_c0	593	69302.16	5.78	-0.371	细胞质 Cytoplasm	-
JsTPS3	c30319.graph_c0	600	69530.43	5.90	-0.351	叶绿体 Chloroplast	-
JsTPS4	c19904.graph_c0	584	66906.02	5.18	-0.326	叶绿体 Chloroplast	-
JsTPS5	c33314.graph_c0	540	62628.94	5.63	-0.245	细胞质 Cytoplasm	α-法尼烯合酶 α-farnesene synthase
JsTPS6	c32177.graph_c0	584	68311.93	5.55	-0.390	叶绿体 Chloroplast	-
JsTPS7	c27401.graph_c0	844	96887.40	6.09	-0.343	细胞质 Cytoplasm	（E,E）-香叶基芳樟醇合酶（E,E）-geranyllinalool synthase
JsTPS8	c44781.graph_c0	163	19103.06	5.64	-0.071	-	-