Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (4): 288-294.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0814
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LI Zhi-kang1(), LIU Chen-xue-xuan1, TAN Chu-min1, XIONG Sheng1,2, XIE Qiu-ling1()
Received:
2021-06-27
Online:
2022-04-26
Published:
2022-05-06
Contact:
XIE Qiu-ling
E-mail:chihong_lai@163.com;txql@jnu.edu.cn
LI Zhi-kang, LIU Chen-xue-xuan, TAN Chu-min, XIONG Sheng, XIE Qiu-ling. Roles and Functions of MFG-E8 as Carrier Proteins in Exosomes[J]. Biotechnology Bulletin, 2022, 38(4): 288-294.
Fig. 1 Structure of MFG-E8-EGFP and its truncated forms as well as gel electrophoresis plots for identification by enzyme digestion A:Schematic diagram of the recombinant plasmids of MFG-E8-EGFP and its truncated forms. B:Gel electrophoresis plots for identifying the recombinant plasmids by enzyme digestion
Fig. 2 Detection of the transient efficiency and expression of the recombinant plasmids A:Detection of the transient efficiency of the recombinant plasmids in HEK293 cells by flow cytometry. B:Detection of target protein expression in HEK293 cells by Western blot. C:Detection of target protein expression in culture supernatant by Western blot
Fig. 4 Transmission electron micrograph and size distribu-tions of exosomes A:Transmission electron micrograph of exosomes,scale bar,100 nm. B:Size distributions of exosomes on NTA measurement
Fig. 5 Identification of EGFP in exosomes A:Western blot detection of MFG-E8-EGFP and its truncated ones in exosomes.B:Transmission electron microscopy images of exosomes derived from HEK293F cell transfected with pCMV-MFG-E8-EGFP(b),pCMV-EC1-EGFP(c)or pCMV-C1C2-EGFP(d). Scale bars,100 nm
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