Cloning and Bioinformatics Analysis of Blue-light Receptor EaWC 1 Gene in Elsinoë arachidis

doi:10.13560/j.cnki.biotech.bull.1985.2021-0937

Abstract

Abstract:

Light is an important information carrier for fungi to perceive and adapt to the environment factors,regulate a variety of physiological and biochemical processes. Elsinochromes（ESC）is an important virulence factor of Elsinoë arachidis causing peanut scab. In order to further reveal mechanism of light regulation on ESC production,gene cloning,bioinformatics analysis and expression of the blue-light receptor gene EaWC 1 were conducted. The results showed that the blue light receptor gene EaWC-1 had a complete reading frame（ORF）with 3 261 bp in length that encoded a protein of 1 086 amino acids. The molecular weight of the protein was 11.95 kD,the theoretical isoelectric point was 8.81. Subcellular localization analysis showed that EaWC-1 was localized in nucleus and was a hydrophilic protein. qPCR analysis indicated that the expression of EaWC 1 gene was positively regulated by blue light,and its expression pattern was significantly and positively correlated with ESC toxin accumulation. This result would provide a theoretical foundation for elucidating the biological functions of blue light receptors of E. arachidis and revealing the photo-regulation mechanism and regulatory network of ESC toxin biosynthesis.

Key words: Elsinoë arachidis, blue-light receptor, gene cloning, bioinformatics analysis, expression analysis

LI Yang, ZHANG Xiao-tian, PIAO Jing-zi, ZHOU Ru-jun, LI Zi-bo, GUAN Hai-wen. Cloning and Bioinformatics Analysis of Blue-light Receptor EaWC 1 Gene in Elsinoë arachidis[J]. Biotechnology Bulletin, 2022, 38(5): 93-99.

Figures/Tables 10

Table 1 Sequence information of WC 1 homologous gene

种名Species	GenBank ID
Elsinoe fawcettii	KAF4549824.1
Elsinoe australis	TKX21094.1
Elsinoe ampelina	KAF2224433.1
Alternaria alternata	QBZ93284.1
Cercospora zeae maydis	AEH41590.1
Pseudocercospora musae	KXT04460.1
Dothistroma septosporum	EME40353.1
Trichoderma atroviride	AAU14171.1
Neurospora crassa	CAA63964.2
Aspergillus nidulans	AAP47230.1
Mucor circinelloides	CAJ13843.2
Phycomyces blakesleeanus	ABB77846.1
Hortaea werneckii	RMY69450.1

Fig. 1 PCR amplification of EaWC 1 gene

Fig.2 Protein domain analysis of WC-1

Fig. 3 Amino acid sequence alignment of WC-1 protein

Fig. 4 Phylogenetic tree of EaWC-1 protein sequence

Fig. 5 Protein hydrophobicity

Fig.6 Analysis of phosphate sites of encoded protein

Fig. 7 Secondary and tertiary structure prediction of protein

Fig. 8 Culture characteristics of E. arachidis

Fig.9 EaWC 1 expression analysis and ESC production light quality

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