Screening,Identification and Broad-spectrum Application of Efficient IAA-producing Bacteria Dissolving Phosphorus and Potassium

doi:10.13560/j.cnki.biotech.bull.1985.2021-1557

Abstract

Abstract:

We screened the growth-promoting bacteria species from plant rhizosphere and found out their growth-promoting ability under laboratory and field conditions,which may provide a potential bacterial fertilizer for promoting the production and efficiency in agriculture generally. The strains were isolated from rhizosphere soil,the IAA production of these strains was determined by Salkowski colorimetry,and the phosphorus- and potassium-dissolving abilities were determined by molybdenum antimony resistance colorimetry and flame photometry. The high-efficiency growth-promoting strains were identified in this study based on the morphology,physiology and biochemistry and 16S rDNA. The optimal conditions of these strains to produce IAA were explored and further applied to the pot and field experiments for studying its actual application effects. A strain of Bacillus wiedmannii YC9 was selected,it produced 61.71 mg/L of IAA,dissolved 17.57 mg/L of potassium and 98.25 mg/L and 0.64 mg/L of inorganic phosphorus and organic phosphorus. The IAA production of YC9 reached the peak under the conditions that the liquid volume was 150/250 mL,the pH was 7-9,the carbon source was glucose,and the nitrogen source was peptone. In the pot experiment,principal component analysis（PCA）combined with correlation analysis indicated that YC9 promoted the growth of tobacco roots and shoots by increasing IAA and available phosphorus and potassium in the soil. In the field trials of tobacco and wheat,the yield of flue-cured tobacco and the proportion of high-quality tobacco increased by 14.3% and 9.6%,respectively,and the yield of wheat increased by 43.8%,indicating that YC9 had a good application effect in both tobacco and wheat cultivation. YC9 has been identified as a multifunctional growth-promoting bacterium that can produce IAA,hydrolyze phosphorus and potassium. It improves the yield and quality of crops efficiently in the fluvo aquic soil. Our study shows it an outstanding microbial resource of growth-promoting bacterial fertilizer with a broad-spectrum application prospect.

Key words: fluvo aquic soil, rhizosphere growth-promoting bacteria, tobacco, wheat, Bacillus wiedmannii

ZHANG Hao-xin, WANG Zhong-hua, NIU bing, GUO Kang, LIU Lu, JIANG Ying, ZHANG Shi-xiang. Screening,Identification and Broad-spectrum Application of Efficient IAA-producing Bacteria Dissolving Phosphorus and Potassium[J]. Biotechnology Bulletin, 2022, 38(5): 100-111.

Figures/Tables 15

References 37

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不同条件Different condition	装液量 Liquid loading/（mL·250 mL^-1）	pH	氮源Nitrogen source	碳源Carbon source
1	25	4	硝酸钾	葡萄糖
2	50	5	硫酸铵	木糖
3	75	6	硝酸铵	蔗糖
4	100	7	酵母粉	果糖
5	150	8	谷氨酸	甘露醇
6		9	尿素	乳糖
7		10	蛋白胨	麦芽糖

不同条件Different condition	装液量 Liquid loading/（mL·250 mL^-1）	pH	氮源Nitrogen source	碳源Carbon source
1	25	4	硝酸钾	葡萄糖
2	50	5	硫酸铵	木糖
3	75	6	硝酸铵	蔗糖
4	100	7	酵母粉	果糖
5	150	8	谷氨酸	甘露醇
6		9	尿素	乳糖
7		10	蛋白胨	麦芽糖

项目 Item	结果 Result	参考菌株 Reference strain	项目 Item	结果 Result	参考菌株 Reference strain
革兰氏染色 Gram stain	+	+	淀粉水解 Amylohydrolysis test	+	+
好氧性试验 Aerobic test	兼性厌氧	兼性厌氧	明胶液化 Gelatin liquefaction test	+	+
接触酶试验 Catalase	+	+	硝酸盐还原 Nitrate reduction test	+	+
甲基红（M.R）反应 Methyl red test	-	-	柠檬酸盐利用 Citrate utilization	-	+（55）
V-P试验 Voges-Proskauer test	+	+

项目 Item	结果 Result	参考菌株 Reference strain	项目 Item	结果 Result	参考菌株 Reference strain
革兰氏染色 Gram stain	+	+	淀粉水解 Amylohydrolysis test	+	+
好氧性试验 Aerobic test	兼性厌氧	兼性厌氧	明胶液化 Gelatin liquefaction test	+	+
接触酶试验 Catalase	+	+	硝酸盐还原 Nitrate reduction test	+	+
甲基红（M.R）反应 Methyl red test	-	-	柠檬酸盐利用 Citrate utilization	-	+（55）
V-P试验 Voges-Proskauer test	+	+

处理Treatment	IAA/（mg·kg^-1）	速效钾Available K/（mg·kg^-1）	速效磷Available P/（mg·kg^-1）
CK	0.26±0.04	135.00±7.35	5.04±0.08
YC9	0.94±0.05^**	149.00±1.73^*	6.11±0.32^*