Nicotinic Acid Promotes γ-aminobutyric Acid Biosynthesis in Lactobacillus brevis

doi:10.13560/j.cnki.biotech.bull.1985.2021-1618

Abstract

Abstract:

Adding 0.15% nicotinic acid to medium significantly increased the γ-aminobutyric acid（GABA）production of Lactobacillus brevis. In order to clarify the role of nicotinic acid，L. brevis was inoculated in the medium with（experimental group）and without nicotinic acid（control group）for fermentation. The differential metabolites were studied by liquid chromatography-mass spectrometry（LC-MS），and the integrity of cell membrane was analyzed using propidium iodide（PI）single staining method. The results showed that 33 metabolites were significantly different between the experimental group and the control group. The unsaturated fatty acids and some amino acids including glutamate，proline，aspartate，serine，histidine，and ornithine，significantly increased in the experimental group，whereas nicotinic acid nucleotide，2-phosphoglycerate，adenosine monophosphate and tyramine significantly decreased. PI single staining experiments demonstrated that the cells with red fluorescence in the experimental group were significantly more than those in the control group. Therefore，the addition of nicotinic acid has a broad impact on the physiological metabolism of L. brevis，and its promoting effect on GABA biosynthesis may be related to increasing the permeability of the cell membrane of L. brevis，resulting in easier substrates entry into the cells and easier products discharge from the cells.

Key words: γ-aminobutyric acid, nicotinic acid, LC-MS, propidium indide

ZHANG Jian, CAI Heng, SHEN Zhen-hao, LIU Zhong-hao, ZHAO Juan, ZHANG Qiao-zhen, GAO Qiang. Nicotinic Acid Promotes γ-aminobutyric Acid Biosynthesis in Lactobacillus brevis[J]. Biotechnology Bulletin, 2022, 38(10): 235-242.

Figures/Tables 4

References 26

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编号No.	代谢物Metabolites	加合离子Addition of ions	调节趋势Trend of change	相关代谢通路Related metabolic pathways
1	组氨酸	（M+H-H2O）⁺	↓*	组氨酸、天冬氨酸代谢
2	二十二碳-13-烯酸	（M+H-H2O）⁺	↑*	脂质代谢
3	脯氨酸	（M+H）⁺	↑**	脯氨酸代谢
4	鸟氨酸	（M+H-H2O）⁺	↑**	精氨酸代谢
5	L-天冬氨酸	（M+H）⁺	↑*	组氨酸、天冬氨酸代谢
6	L-丝氨酸	（M+H）⁺	↑**	丝氨酸代谢
7	脯氨酸-苏氨酸	（M+H）⁺	↑***	二肽
8	脯氨酸-天冬酰胺	（M+NH4）⁺	↑**	二肽
9	二十二碳二烯酸（11（Z），14（Z））	（2M+H）⁺	↑**	脂质代谢
10	氨基己二酸	（M+H-H2O）⁺	↑**	脂质代谢
11	精氨酸-半胱氨酸	（M+CH3CN+H）⁺	↑*	二肽
12	20-羟二十烷四烯酸（20-HETE）	（M-H）^-	↑*	脂质代谢
13	1-棕榈酰2-油酰磷脂酰甘油	（M-H）^-	↑***	脂质代谢
14	谷氨酰胺	（M-H）^-	↑**	谷氨酸代谢
15	D-天冬氨酸	（M-H）^-	↑*	组氨酸、天冬氨酸代谢
16	腺苷一磷酸（AMP）	（M+H）⁺	↓**	嘌呤代谢
17	尿苷一磷酸（UMP）	（M+H）⁺	↓**	嘧啶代谢
18	乙酰胆碱	（M+H）⁺	↓**	脂质代谢
19	6-磷酸甘露糖	（M+H-H2O）⁺	↓***	碳代谢
20	艾杜醇	（M+Na）⁺	↓***	/
21	焦磷酸硫胺素	M⁺	↓**	维生素代谢
22	甜菜碱	（M+H）⁺	↓**	氨基酸代谢
23	单棕榈酸甘油	（M+H-H2O）⁺	↓**	脂质代谢
24	酪胺	（M+H）⁺	↓***	酪氨酸代谢
25	烟酸核苷酸	（M+H）⁺	↓**	烟酸代谢
26	肌醇半乳糖苷	（M+CH3COO）^-	↓**	/
27	植酸	（M-H）^-	↓**	肌醇磷酸代谢
28	2-磷酸甘油酸	（M-H）^-	↓**	糖酵解
29	肌苷酸（IMP）	（M-H2O-H）^-	↓***	嘌呤代谢
30	1-磷酸甘露糖	（M-H）^-	↓***	碳代谢
31	十八碳二烯酸（18：2（9Z，12Z））	（M+H-H2O）⁺	↑**	脂质代谢
32	N-乙酰鸟氨酸	（M+H）⁺	↑*	谷氨酸、鸟氨酸代谢
33	N-乙酰天冬氨酸	（M-H）^-	↑**	谷氨酸、天冬氨酸代谢

编号No.	代谢物Metabolites	加合离子Addition of ions	调节趋势Trend of change	相关代谢通路Related metabolic pathways
1	组氨酸	（M+H-H2O）⁺	↓*	组氨酸、天冬氨酸代谢
2	二十二碳-13-烯酸	（M+H-H2O）⁺	↑*	脂质代谢
3	脯氨酸	（M+H）⁺	↑**	脯氨酸代谢
4	鸟氨酸	（M+H-H2O）⁺	↑**	精氨酸代谢
5	L-天冬氨酸	（M+H）⁺	↑*	组氨酸、天冬氨酸代谢
6	L-丝氨酸	（M+H）⁺	↑**	丝氨酸代谢
7	脯氨酸-苏氨酸	（M+H）⁺	↑***	二肽
8	脯氨酸-天冬酰胺	（M+NH4）⁺	↑**	二肽
9	二十二碳二烯酸（11（Z），14（Z））	（2M+H）⁺	↑**	脂质代谢
10	氨基己二酸	（M+H-H2O）⁺	↑**	脂质代谢
11	精氨酸-半胱氨酸	（M+CH3CN+H）⁺	↑*	二肽
12	20-羟二十烷四烯酸（20-HETE）	（M-H）^-	↑*	脂质代谢
13	1-棕榈酰2-油酰磷脂酰甘油	（M-H）^-	↑***	脂质代谢
14	谷氨酰胺	（M-H）^-	↑**	谷氨酸代谢
15	D-天冬氨酸	（M-H）^-	↑*	组氨酸、天冬氨酸代谢
16	腺苷一磷酸（AMP）	（M+H）⁺	↓**	嘌呤代谢
17	尿苷一磷酸（UMP）	（M+H）⁺	↓**	嘧啶代谢
18	乙酰胆碱	（M+H）⁺	↓**	脂质代谢
19	6-磷酸甘露糖	（M+H-H2O）⁺	↓***	碳代谢
20	艾杜醇	（M+Na）⁺	↓***	/
21	焦磷酸硫胺素	M⁺	↓**	维生素代谢
22	甜菜碱	（M+H）⁺	↓**	氨基酸代谢
23	单棕榈酸甘油	（M+H-H2O）⁺	↓**	脂质代谢
24	酪胺	（M+H）⁺	↓***	酪氨酸代谢
25	烟酸核苷酸	（M+H）⁺	↓**	烟酸代谢
26	肌醇半乳糖苷	（M+CH3COO）^-	↓**	/
27	植酸	（M-H）^-	↓**	肌醇磷酸代谢
28	2-磷酸甘油酸	（M-H）^-	↓**	糖酵解
29	肌苷酸（IMP）	（M-H2O-H）^-	↓***	嘌呤代谢
30	1-磷酸甘露糖	（M-H）^-	↓***	碳代谢
31	十八碳二烯酸（18：2（9Z，12Z））	（M+H-H2O）⁺	↑**	脂质代谢
32	N-乙酰鸟氨酸	（M+H）⁺	↑*	谷氨酸、鸟氨酸代谢
33	N-乙酰天冬氨酸	（M-H）^-	↑**	谷氨酸、天冬氨酸代谢