Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (11): 122-128.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0048
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SUN Rong(), LIU Shan(), GAO Jing-lei
Received:
2022-01-11
Online:
2022-11-26
Published:
2022-12-01
Contact:
LIU Shan
E-mail:sunrong@pzhu.edu.cn;liushan@pzhu.edu.cn
SUN Rong, LIU Shan, GAO Jing-lei. Cloning and Expression Analysis of Flavanone 3-hydroxylase Gene from Bougainvillea spectabilis[J]. Biotechnology Bulletin, 2022, 38(11): 122-128.
Fig. 1 Electrophoresis detection results of RNA extraction and PCR amplification products A:M:D2000 DNA marker;1:RNA sample;B:M:D2000 DNA marker;1:target band
Fig. 2 Alignment analysis of of BsF3H and F3Hs from other plants Underline:2OG-Fe(Ⅱ)oxygenase domain. Red box:Binding site of Fe2+(His215,Asp217,His273). Blue box:Binding site of 2-O-oxoglutarate(Arg283-X-Ser285)
Fig. 7 Analysis of BsF3H expressions in different color B. spectabilis Different lowercase letters above the bars indicate significant differences(P<0.05)among the samples
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