Cloning and Expression Analysis of HbCXXS1，a Thioredoxin Gene in Hevea brasiliensis

doi:10.13560/j.cnki.biotech.bull.1985.2022-0290

Abstract

Abstract:

Thioredoxin plays an important role in plant growth and development and abiotic stress response by regulating cellular redox homeostasis. This study aims to investigate the expressions of thioredoxin gene HbCXXS1 in various tissues of rubber tree（Hevea brasiliensis）and under different hormones and abiotic stresses，for laying a foundation for analyzing its biological function. The HbCXXS1 gene was cloned by RT-PCR and analyzed by bioinformatics. Quantitative real-time PCR was applied to analyze the expression patterns of HbCXXS1 in different tissues of rubber tree and in response to various hormones and abiotic stresses. The results showed that HbCXXS1 contained an open reading frame of 372 bp encoding 123 amino acids. HbCXXS1 was a hydrophilic protein，and contained a conserved thioredoxin domain with a CXXS active site，which belonged to group III of thioredoxin h. HbCXXS1 in the latex was expressed significantly higher than that in other tissues. In addition，its expression was significantly decreased in latex of tapping panel dryness trees as compared with that of healthy trees. The expression of HbCXXS1 was inhibited by abscisic acid，methyl jasmonate，drought and salt stresses but was up-regulated by ethephon and cold stress. Moreover，HbCXXS1 expression was both up-regulated and down-regulated at different time points after salicylic acid and hydrogen peroxide treatments. These results suggested that HbCXXS1 might play an important regulatory role in latex production and latex flow，as well as abiotic stress responses in rubber tree.

Key words: thioredoxin, rubber tree, cloning, expression analysis, abiotic stress

WANG Shuai, YUAN Kun, HE Qi-guang, HU Yi-yu, FENG Cheng-tian, WANG Zhen-hui, LIU Jin-ping, LIU Hui. Cloning and Expression Analysis of HbCXXS1，a Thioredoxin Gene in Hevea brasiliensis[J]. Biotechnology Bulletin, 2022, 38(12): 214-222.

Figures/Tables 6

Fig. 1 PCR amplification of HbCXXS1 gene

Fig. 2 Conserved domain（A），transmembrane domain（B）and hydrophilicity/hydrophobicity（C）analysis of HbCXXS1 protein

Fig. 3 Phylogenetic tree of HbCXXS1 and thioredoxin h proteins in other plants

Fig. 4 Expressions of HbCXXS1 in various tissues（A）and latex from healthy and TPD rubber tree（B） Different lowercase letters indicate a significant difference（P<0.05）. The same below

Fig. 5 Expression change of HbCXXS1 after hormone treatment

Fig. 6 Expression change of HbCXXS1 under abiotic stress

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