Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (6): 149-157.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1275

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GC-MS Analysis and Antioxidant Activities of Liposoluble Components from Three Sanghuangporus Fruit Bodies

LUO Yang-lan1(), CAO Nai-xin2, YANG Yu-mei3, HUANG Li-ling1, YAN Yong1(), HUANG Shi-Lv1   

  1. 1. Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007
    2. Shaanxi University of Technology, Hanzhong 723000
    3. Lueyang County Monitoring and Testing Center of Agricultural Products Quality Safety, Hanzhong 724300
  • Received:2022-10-17 Online:2023-06-26 Published:2023-07-07
  • Contact: YAN Yong E-mail:595059345@qq.com;14544286@qq.com

Abstract:

This work aims to study components and antioxidant activities of liposoluble components from different Sanghuangporus sp. fruiting bodies. Soxhlet extraction method was applied to extract the liposoluble components from different Sanghuangporus sp. fruiting bodies and their chemical compositions were identified by GC-MS. Its reducing ability was determined, and the antioxidant activity was evaluated by the reducing capacity, 1,1-diphenyl 1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging ability and hydroxyl free radical scavenging ability. The GC-MS results suggested that there were 176 identified liposoluble components in total. Liposoluble components of JM1, JM2 and JM3 were 68, 75, 71 compounds, respectively. After classification and analysis, the compounds mainly contained alkane, ester, aromatic group and other substances. There are 8 common components, and their relative contents account for 12.88%, 9.85% and 9.54% of JM1, JM2 and JM3 extracts, respectively, which were 2,4-dimethylheptane, ethylbenzene, 1,4-xylene, styrene, 6-dodecanone, methyl hexadecanoate, methyl tetracosanoate and tetratetracontane. JM1 liposoluble components had the best antioxidant activities. The liposoluble components of three Sanghuangporus sp. fruiting bodies contained a variety of active substances and had various biological activities, which can provide a reference for the further study of their pharmacological efficacy and resource development.

Key words: Sanghuangporus sp., liposoluble components, GC-MS