Biotechnology Bulletin ›› 2019, Vol. 35 ›› Issue (11): 22-29.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0304

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Analyse on Monosaccharide Composition of Polysaccharides in Ziziphus jujuba Mill cv. lingwuchangzao Fruit from Zhongning Areas in Ningxia by GC-MS

ZHANG Ying-cai1, SU Wei-dong2, CHAI Ya-hong1   

  1. 1. College of Life Science,Ningxia University,Yinchuan 750021;
    2. Ningxia jujube engineering technology research center,Lingwu 750400
  • Received:2019-04-11 Online:2019-11-26 Published:2019-11-19

Abstract: The monosaccharide composition of polysaccharides in Ziziphus jujuba Mill cv.lingwuchangzao fruit from Zhongning areas in Ningxia was studied to provide experimental basis for the development and utilization of different areas Ziziphus jujuba Mill cv.lingwuchangzao. The fruit of Ziziphus jujuba Mill cv.lingwuchangzao from Zhongning areas in different developmental periods was used as experimental material to research the change rules of polysaccharides content and monosaccharide composition by the methods of hot water extraction and gas chromatography-mass spectrometry(GC-MS). The main research results are followed:the crude polysaccharides obtaining ratio in Ziziphus jujuba Mill cv. lingwuchangzao fruits from Zhongning areas in different developmental periods is respectively 0.43% in the early bulking period,0.527% in the rapid enlargement period,0.80% in the coloring period,and 0.618% in the fruit maturation period. The crude polysaccharide in different developmental periods fruit was fractioned respectively to four fractions(Ju-0,Ju-1,Ju-2,Ju-3)by DEAE-52 cellulose ion-exchange columns,and Ju-2 had the highest contents,this indicated that the main components of polysaccharides in fruit was acid fractions. Finally,the refined polysaccharides in the different developmental periods fruit were analysed by the GC-MS,the results showed that polysaccharides were composed of ten monosaccharides:arabinose,rhamnose,ribose,fucose,xylose,mannose,galactose,glucose,glucuronic acid and galacturonic acid,and did not contain fructose,the contents of arabinose,galactose,ribose,rhamnose,glucuronic acid and galacturonic acid were higher,followed by the fucose and glucose,and the contents of xylose and mannose were the lowest,but the monosaccharide composition and contents are different in different polysaccharides component. Conclusion,The refined polysaccharide contents present ascendant trend with the development of the fruits,the monosaccharide composition and contents of the refined polysaccharide of Ziziphus jujuba Mill cv.lingwuchangzao fruits from Zhongning areas in different developmental periods are different respectively in different polysaccharides component.

Key words: Zhongning areas, Ziziphus jujuba Mill cv. Lingwuchangzao, fruit, polysaccharides, monosaccharide composition, GC-MS