GC-MS Analysis and Antioxidant Activities of Liposoluble Components from Three Sanghuangporus Fruit Bodies

doi:10.13560/j.cnki.biotech.bull.1985.2022-1275

Abstract

Abstract:

This work aims to study components and antioxidant activities of liposoluble components from different Sanghuangporus sp. fruiting bodies. Soxhlet extraction method was applied to extract the liposoluble components from different Sanghuangporus sp. fruiting bodies and their chemical compositions were identified by GC-MS. Its reducing ability was determined, and the antioxidant activity was evaluated by the reducing capacity, 1,1-diphenyl 1-diphenyl-2-picrylhydrazyl（DPPH）free radical scavenging ability and hydroxyl free radical scavenging ability. The GC-MS results suggested that there were 176 identified liposoluble components in total. Liposoluble components of JM1, JM2 and JM3 were 68, 75, 71 compounds, respectively. After classification and analysis, the compounds mainly contained alkane, ester, aromatic group and other substances. There are 8 common components, and their relative contents account for 12.88%, 9.85% and 9.54% of JM1, JM2 and JM3 extracts, respectively, which were 2,4-dimethylheptane, ethylbenzene, 1,4-xylene, styrene, 6-dodecanone, methyl hexadecanoate, methyl tetracosanoate and tetratetracontane. JM1 liposoluble components had the best antioxidant activities. The liposoluble components of three Sanghuangporus sp. fruiting bodies contained a variety of active substances and had various biological activities, which can provide a reference for the further study of their pharmacological efficacy and resource development.

Key words: Sanghuangporus sp., liposoluble components, GC-MS

LUO Yang-lan, CAO Nai-xin, YANG Yu-mei, HUANG Li-ling, YAN Yong, HUANG Shi-Lv. GC-MS Analysis and Antioxidant Activities of Liposoluble Components from Three Sanghuangporus Fruit Bodies[J]. Biotechnology Bulletin, 2023, 39(6): 149-157.

Figures/Tables 8

Fig. 1 Morphological characteristics of strain JM1 A：Fruiting body. B：Colony. C：Hyphae. The same below

Fig. 2 Morphological characteristics of strain JM2

Fig. 3 Morphological characteristics of strain JM3

Fig. 4 Phylogenetic tree of strain based on the ITS sequences

Fig. 5 GC-MS profile of liposoluble components A, B and C are the GC-MS analysis of JM1, JM2 and JM3 liposoluble components respectively

Fig. 6 Reducing capacities of liposoluble components from different Sanghuangporus sp. fruiting bodies

Fig. 7 DPPH free radical scavenging abilities of liposoluble components from different Sanghuangporus sp. fruiting bodies

Fig. 8 Hydroxyl radical scavenging abilities of liposoluble components from different Sanghuangporus sp. fruiting bodies

References 37

[1]	Wu F, Zhou LW, Vlasák J, et al. Global diversity and systematics of Hymenochaetaceae with poroid hymenophore[J]. Fungal Divers, 2022, 113(1): 1-192. doi: 10.1007/s13225-021-00496-4
[2]	吴声华, 戴玉成. 药用真菌桑黄的种类解析[J]. 菌物学报, 2020, 39(5): 781-794.
	Wu SH, Dai YC. Species clarification of the medicinal fungus Sanghuang[J]. Mycosystema, 2020, 39(5): 781-794.
[3]	王豪, 钱坤, 司静, 等. 桑黄类真菌多糖研究进展[J]. 菌物学报, 2021, 40(4): 895-911.
	Wang H, Qian K, Si J, et al. Research advances on polysaccharides from Sanghuang[J]. Mycosystema, 2021, 40(4): 895-911.
[4]	Lin WC, Deng JS, Huang SS, et al. Anti-inflammatory activity of Sanghuangporus sanghuang mycelium[J]. Int J Mol Sci, 2017, 18(2): E347.
[5]	边亮, 陈华国, 周欣. 植物多糖的抗肿瘤活性研究进展[J]. 食品科学, 2020, 41(7): 275-282. doi: 10.7506/spkx1002-6630-20190330-392
	Bian L, Chen HG, Zhou X. Recent advances in understanding the antitumor activity of polysaccharides from plants[J]. Food Sci, 2020, 41(7): 275-282.
[6]	Cheng JW, Song JL, Wei HL, et al. Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia[J]. Int J Biol Macromol, 2020, 164: 3305-3314. doi: 10.1016/j.ijbiomac.2020.08.202 URL
[7]	张健, 侯玉浩, 杜继甫, 等. 桑树桑黄、瓦尼桑黄和粗毛纤孔菌免疫调节作用的比较研究[J]. 菌物学报, 2023, 42(4):907-915.
	ZHANG J, HOU YH, DU JF, et al. A comparative study on immunomodulatory effects of water extracts of Inonotus hispidus, Sanghuangporus sanghuang and S. vaninii[J]. Mycosystema, 2023, 42(4): 907-915.
[8]	孙波, 周洪英, 吴洪丽, 等. 湖北夷陵野生桑树桑黄生境调查[J]. 中国食用菌, 2021, 40(10): 18-24.
	Sun B, Zhou HY, Wu HL, et al. Habitat investigation of wild Sanghuangporus sanghuang in Yiling of Hubei Province[J]. Edible Fungi China, 2021, 40(10): 18-24.
[9]	付立忠, 陆娜, 闫静, 等. 三种桑黄属真菌人工栽培子实体营养、药效成分及抗氧化活性分析评价[J]. 菌物学报, 2021, 40(8): 2148-2158.
	Fu LZ, Lu N, Yan J, et al. Analyses and evaluation of nutrition, active component and antioxidant activities of fruiting bodies of three species of Sanghuangporus[J]. Mycosystema, 2021, 40(8): 2148-2158.
[10]	李乐. 桑黄活性多糖分离纯化、结构鉴定及体内生物活性研究[D]. 西安: 陕西师范大学, 2015.
	Li L. Purification, structure identification and biological activity in vivo of active polysaccharides from Phellinus igniarius[D]. Xi'an: Shaanxi Normal University, 2015.
[11]	张敬. 杨树桑黄多糖对心肌细胞损伤保护作用研究及杨树桑黄产品开发[D]. 长春: 吉林农业大学, 2021.
	Zhang J. Study on the protective effect of polysaccharides Phellinus vaninii Ljub on myocardial cell injury and development of Phellinus vaninii Ljub products[D]. Changchun: Jilin Agricultural University, 2021.
[12]	王超儀, 包海鹰. 6种“桑黄”石油醚提取物的体内抗肿瘤活性[J]. 菌物研究, 2013, 11(3): 196-201.
	Wang CY, Bao HY. Study on antitumor effect of petroleum ether extracts from fruiting body of six kinds of “Sanghuang” on hepatoma H22 cell[J]. J Fungal Res, 2013, 11(3): 196-201.
[13]	杨焱, 陈晓华, 戴玉成, 等. 我国桑黄产业发展现状、问题及展望──桑黄产业发展千岛湖宣言[J]. 菌物学报, 2023, 42(4):855-873.
	YANG Y, CHEN Xh, DAI YC, et al. Sanghuang industry in China: current status, challenges and perspectives—The Qiandao Lake declaration for sanghuang industry development[J]. Mycosystema, 2023, 42(4):855-873.
[14]	王寿南, 陈青君, 张国庆, 等. 一株野生桑黄的分离鉴定与生物学特性[J]. 应用与环境生物学报, 2016, 22(4): 667-674.
	Wang SN, Chen QJ, Zhang GQ, et al. Isolation, identification, and biological characterization of a wild Sanghuang mushroom[J]. Chin J Appl Environ Biol, 2016, 22(4): 667-674.
[15]	罗阳兰, 邓百万, 刘军生, 等. 秦巴山区蕙兰内生真菌多样性分析[J]. 河南农业科学, 2019, 48(5): 113-121.
	Luo YL, Deng BW, Liu JS, et al. Diversity analysis of endophytic fungi isolated from Cymbidium faberi in Qin-Ba mountains[J]. J Henan Agric Sci, 2019, 48(5): 113-121.
[16]	刘军生, 解修超, 罗阳兰, 等. Aspergillus sp. EA-LJS80的分离及其发酵液提取物七叶皂苷C的生物活性[J]. 菌物学报, 2018, 37(1): 79-87.
	Liu JS, Xie XC, Luo YL, et al. Escin C from Aspergillus sp. EA-LJS80 and its biological activities[J]. Mycosystema, 2018, 37(1): 79-87.
[17]	王寒, 罗庆华, 魏梦雅, 等. 大鲵油体外抗氧化活性研究[J]. 中国油脂, 2018, 43(9): 149-153.
	Wang H, Luo QH, Wei MY, et al. In vitro antioxidant activity of Chinese giant salamander oil[J]. China Oils Fats, 2018, 43(9): 149-153.
[18]	罗阳兰, 邓百万, 刘军生, 等. 白及花与块茎石油醚部位成分分析及其生物活性的比较研究[J]. 中国现代应用药学, 2019, 36(4): 444-450.
	Luo YL, Deng BW, Liu JS, et al. Comparative research of bioactivity and analysis of petroleum ether components in flower and tuber of Bletilla striata[J]. Chin J Mod Appl Pharm, 2019, 36(4): 444-450.
[19]	牛新奎, 韩小贤, 郑学玲. 黑小麦粉的抗氧化成分及活性[J]. 现代食品科技, 2022, 38(9): 292-297.
	Niu XK, Han XX, Zheng XL. Antioxidant components and activity of Triticum aestivum flour[J]. Mod Food Sci Technol, 2022, 38(9): 292-297.
[20]	何晋浙, 徐瑶阳, 孙培龙. 不同来源猴头菌营养成分及其多糖化学组成和抗氧化活性比较[J]. 食品与发酵工业, 2016, 42(1): 134-140.
	He JZ, Xu YY, Sun PL. The comparison of nutrients, chemical composition and antioxidant activity of polysaccharides from six Hericium erinaceus specimens[J]. Food Ferment Ind, 2016, 42(1): 134-140.
[21]	吕国英, 宋婷婷, 蔡为明, 等. 野生桑树桑黄和杨树桑黄化学成分及抗氧化活性比较[J]. 菌物学报, 2021, 40(7): 1833-1843.
	Lv GY, Song TT, Cai WM, et al. Comparative study of chemical components and antioxidant Sanghuang and Sanghuangporus vaninii[J]. Mycosystema, 2021, 40(7): 1833-1843.
[22]	Wen CT, Zhang JX, Zhang HH, et al. Plant protein-derived antioxidant peptides: Isolation, identification, mechanism of action and application in food systems: a review[J]. Trends Food Sci Technol, 2020, 105: 308-322. doi: 10.1016/j.tifs.2020.09.019 URL
[23]	Lorenzo JM, et al. Bioactive peptides as natural antioxidants in food products - A review[J]. Trends Food Sci Technol, 2018, 79: 136-147. doi: 10.1016/j.tifs.2018.07.003 URL
[24]	游庆红, 陈梅琳, 赵增东, 等. 响应面法优化酶解草菇蛋白制备抗氧化肽工艺[J]. 食品研究与开发, 2022, 43(4): 149-157.
	You QH, Chen ML, Zhao ZD, et al. Optimization by response surface methodology of enzymatic hydrolysis of Volvariella volvace protein to obtain antioxidant peptides[J]. Food Res Dev, 2022, 43(4): 149-157.
[25]	王晓敏, 史冠莹, 王赵改, 等. 不同产地香椿抗氧化活性及挥发性成分的差异分析[J]. 现代食品科技, 2020, 36(7): 271-281.
	Wang XM, Shi GY, Wang ZG, et al. Differences in the antioxidant activities and volatile components of Toona sinensis from different regions[J]. Mod Food Sci Technol, 2020, 36(7): 271-281.
[26]	谭小青, 刘布鸣, 黄艳, 等. 圆果算盘子脂溶性成分及其抗氧化活性研究[J]. 中国现代应用药学, 2021, 38(2): 196-200.
	Tan XQ, Liu BM, Huang Y, et al. Study on liposoluble constituents from Glochidion sphaerogynum(muell. arg.)kurz and its antioxidant activities[J]. Chin J Mod Appl Pharm, 2021, 38(2): 196-200.
[27]	乔璐, 栗磊, 宋梦娇, 等. 石胆草脂溶性成分GC-MS分析及其抗氧化活性研究[J]. 中国医药科学, 2020, 10(17): 78-81.
	Qiao L, Li L, Song MJ, et al. A GC-MS analysis of liposoluble components of herb of fanshaped Corallodiscus and a study of the antioxidative activity[J]. China Med Pharm, 2020, 10(17): 78-81.
[28]	周琪琪. 有机酸、癸酸和地衣芽孢杆菌对感染大肠杆菌K88的仔猪的保护作用[D]. 广州: 华南农业大学, 2018.
	Zhou QQ. Protective effects of organic acids, capric acid and Bacillus licheniformis on piglets infected with Escherichia coli K88[D]. Guangzhou: South China Agricultural University, 2018.
[29]	崔承远. 婴幼儿配方奶粉中丁酸、己酸、辛酸、癸酸的测定方法验证[J]. 中国乳业, 2021(12): 105-110.
	Cui CY. Validation of determination method of butyric acid, caproic acid, octanoic acid and capric acid in infant formula milk powder[J]. China Dairy, 2021(12): 105-110.
[30]	张新蕊, 王祝年, 王茂媛, 等. 猪屎豆种子脂溶性成分及其抗氧化活性研究[J]. 热带作物学报, 2011, 32(9): 1669-1672.
	Zhang XR, Wang ZN, Wang MY, et al. Study on the components and antioxidant activities of liposoluble extract from Crotalaria pallida seed[J]. Chin J Trop Crops, 2011, 32(9): 1669-1672.
[31]	徐晶. 香樟和泽漆有效成分的分离分析及活性研究[D]. 东营: 中国石油大学(华东), 2015.
	Xu J. Studies on two medicinal plants Cinnamomum camphora and Euphorbia helioscopia L.: chemistry and bioactivity[D]. Dongying: China University of Petroleum(Huadong), 2015.
[32]	王威, 曹翠平, 陈颖, 等. 棕榈酸诱导胰岛素瘤细胞MIN6细胞凋亡[J]. 基础医学与临床, 2010, 30(4): 401-405.
	Wang W, Cao CP, Chen Y, et al. Palmitate induces cell apoptosis in insulinoma MIN₆ cell[J]. Basic ＆ Clin Med, 2010, 30(4): 401-405.
[33]	谢珊珊, 朱文博, 颜敏, 等. 3β, 5α, 6β-三羟基胆甾烷诱导恶性胶质瘤细胞的凋亡[J]. 中国病理生理杂志, 2013, 29(4): 647-653.
	Xie SS, Zhu WB, Yan M, et al. Cholestane-3β, 5α, 6β-triol induces apoptosis of malignant glioma cells[J]. Chin J Pathophysiol, 2013, 29(4): 647-653.
[34]	胥莉. 亚油酸氧化产物的体外活性和促炎作用[D]. 杨凌: 西北农林科技大学, 2013.
	Xu L. The study on in vitro bioactivity of linoleic acid oxidation product and its pro-inflammatory effect[D]. Yangling: Northwest A & F University, 2013.
[35]	黄周锋, 卢文杰, 谭晓, 等. 裸花水竹草脂溶性成分及其抗氧化活性研究[J]. 中国现代应用药学, 2018, 35(5): 674-677.
	Huang ZF, Lu WJ, Tan X, et al. Study on the components and antioxidant activites of the liposoluble constituents from Murdannia nudiflora(linn.) brenan[J]. Chin J Mod Appl Pharm, 2018, 35(5): 674-677.
[36]	高雪峰, 韩国栋. 根分泌物2, 4-二叔丁基苯酚对短花针茅荒漠草原土壤细菌群落的影响[J]. 生态环境学报, 2019, 28(9): 1731-1738. doi: 10.16258/j.cnki.1674-5906.2019.09.003
	Gao XF, Han GD. Effects of root exudate 2, 4-bis(1, 1-dimethylethyl)-phenol on soil bacterial community in Stipa breviflora desert steppe[J]. Ecol Environ Sci, 2019, 28(9): 1731-1738.
[37]	马新欣, 权乾坤, 田苑, 等. 帕罗西汀治疗阿尔兹海默症合并抑郁对血清NE以及5-HT表达的影响[J]. 现代生物医学进展, 2020, 20(21): 4080-4083.
	Ma XX, Quan QK, Tian Y, et al. Effects of paroxetine on Alzheimer's disease combined with depression on serum NE and 5-HT expression[J]. Prog Mod Biomed, 2020, 20(21): 4080-4083.