Control Ability and Identification of Biocontrol Agent HK11-9 Against Corynespora Leaf Spot of Cucumber

doi:10.13560/j.cnki.biotech.bull.1985.2023-0407

Abstract

Abstract:

In order to explore the biocontrol ability of biocontrol agent HK11-9 against Corynespora leaf spot of cucumber, the antifungal activity of HK11-9 against Corynespora cassiicola were detected by in petri dishes experiments, the control effect of HK11-9 against Corynespora leaf spot of cucumber were detected by in vitro and in vivo inoculation experiments, the colonization ability of HK11-9 on cucumber leaves and its effect on the activities of defense enzymes were conducted by pot experiment, at the same time, the biological functions of HK11-9 was determined. The results showed that, HK11-9 significantly inhibited the mycelia growth and conidia germination of Corynespora cassiicola in the petri dishes experiment, HK11-9 presented significant control effect against Corynespora leaf spot of cucumber at the in vitro and in vivo inoculation experiment, the control efficiencies were 62.80% and 67.73% respectively. HK11-9 colonized stably in cucumber leaves, maintained a density of 10⁴CFU/g in cucumber leaves, and HK11-9 significantly improved the activities of defense enzymes in cucumber leaves. In addition, HK11-9 decomposed protein, cellulose and starch, produced siderophore, and HK11-9 demonstrated antifungal activity against a variety plant pathogenic fungi. Based on the morphological, physiological characteristics and 16S rRNA sequence analysis, the strain HK11-9 was identified as Paenibacillus polymyxa. In summary, the biocontrol agent HK11-9 has great potential to control Corynespora leaf spot of cucumber.

Key words: Corynespora leaf spot of cucumber, Paenibacillus polymyxa HK11-9, colonization density, activity of defense enzyme, identification, biological control

ZHANG Lin-lin, SHEN Hu-sheng, YANG Bing, HE Meng-han, PIAO Feng-zhi, SHEN Shun-shan. Control Ability and Identification of Biocontrol Agent HK11-9 Against Corynespora Leaf Spot of Cucumber[J]. Biotechnology Bulletin, 2023, 39(12): 209-218.

Figures/Tables 14

References 38

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处理 Treatment	离体接种 In vitro inoculation		活体接种In vivo inoculation
处理 Treatment	病情指数Disease index	防治效果Control effect/%	病情指数Disease index	防治效果Control effect/%
HK11-9	22.03±1.13a	62.80	21.17±1.67a	67.73
化学药剂Pesticides	24.21±0.97a	59.12	25.25±2.33a	61.52
对照Control	59.23±1.33b		65.61±2.21b

处理 Treatment	离体接种 In vitro inoculation		活体接种In vivo inoculation
处理 Treatment	病情指数Disease index	防治效果Control effect/%	病情指数Disease index	防治效果Control effect/%
HK11-9	22.03±1.13a	62.80	21.17±1.67a	67.73
化学药剂Pesticides	24.21±0.97a	59.12	25.25±2.33a	61.52
对照Control	59.23±1.33b		65.61±2.21b

处理 Treatment	病情指数 Disease index	防治效果 Control effect/%
菌悬液Bacteria suspension	24.38±0.87a	66.35
发酵液Fermentation liquid	25.50±1.12a	64.80
上清液Fermentation solution	69.96±2.04b	3.44
对照 Control	72.45±1.67b

处理 Treatment	病情指数 Disease index	防治效果 Control effect/%
菌悬液Bacteria suspension	24.38±0.87a	66.35
发酵液Fermentation liquid	25.50±1.12a	64.80
上清液Fermentation solution	69.96±2.04b	3.44
对照 Control	72.45±1.67b

鉴定指标Identification index	结果 Result
菌体形态Cell morphology	直杆状Straight rod
菌体大小Cell size	（0.5-0.8）μm×（2.4-2.8）μm
鞭毛Flagellum	周生Multi flagella
革兰氏染色Gram reaction	G+
耐盐度Salt concentration	≤4%
生长温度Growth temperature	4-50℃
最适生长温度 Optimal growth temperature	30℃
运动性Motility	+
生长所需营养 Nutrition
蔗糖Sucrose	+
葡萄糖Glucose	+
甘油Glycerol	+
麦芽糖Maltose	+
半乳糖Galactose	-
单宁酸Tannin	-
甘露醇Mannitol	+
柠檬酸Citric acid	-
硝酸盐还原Nitrate reduction	-
亚硝酸还原nitrous acid	+
硫化氢Hydrogen sulfide	-
明胶液化Gelatin liquefaction	-
淀粉水解Amylolysis	+
葡萄糖氧化Glucose oxidative	发酵型 Fermentation