Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (12): 148-157.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0819

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Transcriptome Analysis of Cucumber Seeds Early and Late Germination Under PEG Drought Simulation

XIE Yang(), ZHOU Guo-yan, SU Hang, XING Yu-meng, YAN Li-ying   

  1. College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Hebei Higher Institute Application Technology Research and Development Center of Horticultural Plant Biological Breeding, Qinhuangdao 066004
  • Received:2023-08-20 Online:2023-12-26 Published:2024-01-11
  • Contact: XIE Yang E-mail:xieyangly123@163.com

Abstract:

Drought is one of the most common abiotic stress factors that affect the growth and development of cucumber and reduce the yield. To analyze the molecular mechanism of cucumber drought stress response, the seeds of drought resistant cucumber ‘KS33’and drought sensitive cucumber ‘KS30’ were treated with PEG drought simulation, and transcriptomic sequencing was performed at early and late germination stages. The results showed that early germination was an important database for drought resistance gene mining, among which 727 up-regulated and 345 down-regulated differential genes were identified in PEG drought simulation induction of drought-resistant materials in early germination compared with the control(DT1_TvsDT1_CK). Compared with the control(DS1_TvsDS1_CK), 1 226 up-regulated and 111 down-regulated differential genes were identified by PEG drought simulation induction during early germination of sensitive materials.Via KEGG enrichment analysis the 20 key drought resistance candidate genes were identified to be enriched in linoleic acid metabolism(csv00591), pentose, glucuronic acid conversion(csv00040), phenylpropanoid biosynthesis(csv00940)and plant hormone signal transduction(csv04075)pathways. The drought-resistant gene CsaV3_4G023820, CsaV3_2G006420 and two related SNP molecular markers were selected by associated SNP mutation site and RT-qPCR analysis.

Key words: cucumber, drought, transcriptome, molecular markers