Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (2): 130-145.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0869
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WU Cui-cui1,3(), XIAO Shui-ping2,3()
Received:
2023-09-07
Online:
2024-02-26
Published:
2024-03-13
Contact:
XIAO Shui-ping
E-mail:wucuicui19821021@126.com;shuipingxiao@163.com
WU Cui-cui, XIAO Shui-ping. Genome-wide Identification of HD-Zip Gene Family in Gossypium hirsutum L. and Expression Analysis in Response to Abiotic Stress[J]. Biotechnology Bulletin, 2024, 40(2): 130-145.
基因名称Gene name | 正向引物Forward primer(5'-3') | 反向引物Reverse primer(5'-3') |
---|---|---|
GhHis3 | F:CGGTGGTGTGAAGAAGCCTCAT | R:AATTTCACGAACAAGCCTCTGGAA |
GhHDZ12 | F:CAGGCAAAGCAAAGTGGAGA | R:AGATCTGCAGCTGGTTCACT |
GhHDZ15 | CGGGTGGGCACAAAATAAGT | TTGGTTTCAGAAATGGGGCC |
GhHDZ46 | F:CCACCACAAAAGCCTGTTGA | R:GGTCGGAGTAATCATTGCCT |
GhHDZ50 | F:CCAGCAAGTTTTTAGGGCGA | R:GGAACCACCGGCAAAGAATT |
GhHDZ76 | F:ATGCTGAGCTCCAAGTCCTT | R:ATCTTGCACAACACAGCCAG |
GhHDZ116 | F:GGCCCCATTTCTGAAACCAA | R:TGGCGACTCAACTTCTGTCT |
GhHDZ119 | F:ACCAGCTGCAGATCTCAACT | R:CCCTTGTTGGTGTTGGTGTT |
GhHDZ146 | F:CCACCACAAAAGCCTGTTGA | R:CGGGTCGGAGTAATCATTGT |
GhHDZ176 | F:ATGCTGAGCTCCAAGTCCTT | R:ATCTTGCACAACACAGCCAG |
GhHDZ188 | F:ACGTCGCACTTTTACCTTCG | R:ACCGTTTCAACCGATTCGAC |
GhHDZ146-sub | F:TTGATACATATGCCCGTCGACATG | R:GTCGTGGTCCTTATAGTCGGATCCAT |
GCGGGTGGGAGGGTCTTCTCTTGTAA | AAGCCGAAGACCAGAAAGCATGATCTTC |
Table 1 Primers used in the study
基因名称Gene name | 正向引物Forward primer(5'-3') | 反向引物Reverse primer(5'-3') |
---|---|---|
GhHis3 | F:CGGTGGTGTGAAGAAGCCTCAT | R:AATTTCACGAACAAGCCTCTGGAA |
GhHDZ12 | F:CAGGCAAAGCAAAGTGGAGA | R:AGATCTGCAGCTGGTTCACT |
GhHDZ15 | CGGGTGGGCACAAAATAAGT | TTGGTTTCAGAAATGGGGCC |
GhHDZ46 | F:CCACCACAAAAGCCTGTTGA | R:GGTCGGAGTAATCATTGCCT |
GhHDZ50 | F:CCAGCAAGTTTTTAGGGCGA | R:GGAACCACCGGCAAAGAATT |
GhHDZ76 | F:ATGCTGAGCTCCAAGTCCTT | R:ATCTTGCACAACACAGCCAG |
GhHDZ116 | F:GGCCCCATTTCTGAAACCAA | R:TGGCGACTCAACTTCTGTCT |
GhHDZ119 | F:ACCAGCTGCAGATCTCAACT | R:CCCTTGTTGGTGTTGGTGTT |
GhHDZ146 | F:CCACCACAAAAGCCTGTTGA | R:CGGGTCGGAGTAATCATTGT |
GhHDZ176 | F:ATGCTGAGCTCCAAGTCCTT | R:ATCTTGCACAACACAGCCAG |
GhHDZ188 | F:ACGTCGCACTTTTACCTTCG | R:ACCGTTTCAACCGATTCGAC |
GhHDZ146-sub | F:TTGATACATATGCCCGTCGACATG | R:GTCGTGGTCCTTATAGTCGGATCCAT |
GCGGGTGGGAGGGTCTTCTCTTGTAA | AAGCCGAAGACCAGAAAGCATGATCTTC |
Fig. 3 Syntenic relationship of GhHDZ gene family A01-A13 represent the A subgroup chromosomes of upland cotton, and D01-D13 represent the D subgroup chromosomes of upland cotton
Fig. 4 Phylogenetic, gene structure, and motif analyses of GhHDZ family a: Phylogenetic relationships between GhHDZ members are based on the full-length protein sequences. b: Gene motif analysis of GhHDZ family. c: Gene structure analysis of GhHDZ family. Gene structure maps were drawn with GSDS. Green boxes indicated exons; grey lines indicated introns. The scale bar is shown at the bottom
Fig. 9 Salt stress identification of GhHDZ146 overexpression in Arabidopsis thaliana a and b: Comparison of root length between GhHDZ146-OE and WT. Bar=1 cm. c and d: Comparison of root hair length between GhHDZ146-OE and WT. Bar= 50 μm. The difference was analyzed by double-tailed t-test. The error line represents the average ±SD. Among them, WT represents wild type and OE represents GhHDZ146 overexpression strain. * P < 0.05,** P < 0.01
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