Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (6): 161-171.doi: 10.13560/j.cnki.biotech.bull.1985.2023-1153
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LIU Rong1,2(), TIAN Min-yu1,2, LI Guang-ze1,2, TAN Cheng-fang2, RUAN Ying2, LIU Chun-lin1,2()
Received:
2023-12-08
Online:
2024-06-26
Published:
2024-05-23
Contact:
LIU Chun-lin
E-mail:2804035191@qq.com;liucl@hunau.edu.cn
LIU Rong, TIAN Min-yu, LI Guang-ze, TAN Cheng-fang, RUAN Ying, LIU Chun-lin. Identification and Induced-expression Analysis of REVEILLE Family in Brassica napus L.[J]. Biotechnology Bulletin, 2024, 40(6): 161-171.
Gene name | Forword primer sequence(5'-3') | Reverse primer sequence(5'-3') |
---|---|---|
BnaRVE1d | TGTCTCCTGTTTCCTTTGCATCACC | AACTCTCTCTGTTTACGAGTCTCTCTCT |
BnaRVE8b | TTCGACCTCCGGTATGGGAAG | GAATTACCTTGAAGCACCGGAGG |
BnaRVE3c | GTGTGGCTCTTACAACTTCAAACGCAT | CACCCAATTCCTCTTTGATCACTGGAA |
BnaRVE5b BnaActin2 | TCTCCACTTATTCGATCGAGATTGGAAG GTCTTCTCTGCTCTTCTCA | GTTCGTTAGCACCGCTCTTCTG CCATTCCAGTTCCATTGTC |
Table 1 Real-time fluorescent quantitative primers for RVE gene family expression analysis in B. napus
Gene name | Forword primer sequence(5'-3') | Reverse primer sequence(5'-3') |
---|---|---|
BnaRVE1d | TGTCTCCTGTTTCCTTTGCATCACC | AACTCTCTCTGTTTACGAGTCTCTCTCT |
BnaRVE8b | TTCGACCTCCGGTATGGGAAG | GAATTACCTTGAAGCACCGGAGG |
BnaRVE3c | GTGTGGCTCTTACAACTTCAAACGCAT | CACCCAATTCCTCTTTGATCACTGGAA |
BnaRVE5b BnaActin2 | TCTCCACTTATTCGATCGAGATTGGAAG GTCTTCTCTGCTCTTCTCA | GTTCGTTAGCACCGCTCTTCTG CCATTCCAGTTCCATTGTC |
Fig. 5 Intra-specic collinearity analysis of B. napus RVE family(A)and collinearity analysis of RVE family genes among A. thaliana, B. napus, B. rapa and B. oleracea(B-D)
Fig. 7 RT-qPCR expression analysis of BnaRVE under different stress treatments A: Expression of BnaRVE in MeJA processing.B: Expression of BnaRVE in ABA processing. C: Expression of BnaRVE in cold treatment
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