Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (6): 180-189.doi: 10.13560/j.cnki.biotech.bull.1985.2023-1171
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ALIYA Waili1(), CHEN Yong-kun1, KELAREMU Kelimujiang1, WANG Bao-qing2(), CHEN Ling-na1()
Received:
2023-12-13
Online:
2024-06-26
Published:
2024-05-23
Contact:
WANG Bao-qing, CHEN Ling-na
E-mail:2090926128@qq.com;309114577@qq.com;ln.chen@xjnu.edu.cn
ALIYA Waili, CHEN Yong-kun, KELAREMU Kelimujiang, WANG Bao-qing, CHEN Ling-na. Phylogenetic Evolution and Expression Analysis of SPL Gene Family in Juglans regia[J]. Biotechnology Bulletin, 2024, 40(6): 180-189.
软件或在线工具名称Name of the software or online tool | 用途Usage |
---|---|
ProtParam在线工具( | 蛋白理化性质预测Prediction of protein physicochemical properties |
ProtComp9.0( | 亚细胞定位预测Prediction of subcellular localization |
MEME程序( | 保守基序分析Conservative motif analysis |
ClustalW2(v2.1) | 多重序列比对Multiple sequence alignment |
PlantCARE( | 启动子顺式作用元件预测Prediction of promoter cis-acting elements |
Table 1 Bioinformatics software and purpose of usage
软件或在线工具名称Name of the software or online tool | 用途Usage |
---|---|
ProtParam在线工具( | 蛋白理化性质预测Prediction of protein physicochemical properties |
ProtComp9.0( | 亚细胞定位预测Prediction of subcellular localization |
MEME程序( | 保守基序分析Conservative motif analysis |
ClustalW2(v2.1) | 多重序列比对Multiple sequence alignment |
PlantCARE( | 启动子顺式作用元件预测Prediction of promoter cis-acting elements |
引物名称 Primer name | 引物序列 Primer sequence(5'-3') | 引物名称 Primer name | 引物序列 Primer sequence(5'-3') | |
---|---|---|---|---|
JrSPL1-F | ATCCCCCTCGCAATGGGAGTGG | JrSPL16-R | CCTCCAAAGGCTTGTTCTTCTCCGC | |
JrSPL1-R | CAGAGAAGCATGTCCCAAGTCTGG | JrSPL20-F | GCAGAGCCGAGGGAAAGAGGAG | |
JrSPL2-F | ATCCCCCTCGCAATGGGAGTGG | JrSPL20-R | CCGCCTGGCAAGTAATCAGCGTTG | |
JrSPL2-R | CAGAGAAGCATGTCCCAAGTCTGG | JrSPL21-F | CGCCGATGCCAAGCCGTACC | |
JrSPL3-F | CTCTCTGGCCGAGTCGGGAG | JrSPL21-R | GTCGCCTCTCATTGTGTCCGGC | |
JrSPL3-R | CACCACTCCTCACCTTCTTCGGC | JrSPL22-F | CTCTCTTCTGTCATCACCTCCGGC | |
JrSPL4-F | CGTCTGCTTCTCCACCCATCTCG | JrSPL22-R | CGGATCCCATGTGAAACCTTCCGC | |
JrSPL4-R | CAGCACACCACCACCCCTCACC | JrSPL23-F | GTGCCAGGTGTGCAGTTGTTGCAAG | |
JrSPL8-F | ACAGGCCGGATGCCATAGTCGAG | JrSPL23-R | CCCACTTGAATGGGGCAACCGAG | |
JrSPL8-R | CTGCCTGGCAACATGGCGGTG | JrSPL25-F | GCTATGAGTGCTGCGGATTTGCGG | |
JrSPL14-F | GGCTCGTGGTGTGGCAGTTCATC | JrSPL25-R | TTGAAGAGCCCCCGGTCAGAGG | |
JrSPL14-R | CTGAGGGTGTAGGGACTCGGGA | Jractin-F | ATGATGTCAAGGAAGGACTC | |
JrSPL16-F | GGCACCGCAGCGAATGATGACAC | Jractin-R | CACAATGATCTCAGCTCCG |
Table 2 RT-qPCR primer sequence
引物名称 Primer name | 引物序列 Primer sequence(5'-3') | 引物名称 Primer name | 引物序列 Primer sequence(5'-3') | |
---|---|---|---|---|
JrSPL1-F | ATCCCCCTCGCAATGGGAGTGG | JrSPL16-R | CCTCCAAAGGCTTGTTCTTCTCCGC | |
JrSPL1-R | CAGAGAAGCATGTCCCAAGTCTGG | JrSPL20-F | GCAGAGCCGAGGGAAAGAGGAG | |
JrSPL2-F | ATCCCCCTCGCAATGGGAGTGG | JrSPL20-R | CCGCCTGGCAAGTAATCAGCGTTG | |
JrSPL2-R | CAGAGAAGCATGTCCCAAGTCTGG | JrSPL21-F | CGCCGATGCCAAGCCGTACC | |
JrSPL3-F | CTCTCTGGCCGAGTCGGGAG | JrSPL21-R | GTCGCCTCTCATTGTGTCCGGC | |
JrSPL3-R | CACCACTCCTCACCTTCTTCGGC | JrSPL22-F | CTCTCTTCTGTCATCACCTCCGGC | |
JrSPL4-F | CGTCTGCTTCTCCACCCATCTCG | JrSPL22-R | CGGATCCCATGTGAAACCTTCCGC | |
JrSPL4-R | CAGCACACCACCACCCCTCACC | JrSPL23-F | GTGCCAGGTGTGCAGTTGTTGCAAG | |
JrSPL8-F | ACAGGCCGGATGCCATAGTCGAG | JrSPL23-R | CCCACTTGAATGGGGCAACCGAG | |
JrSPL8-R | CTGCCTGGCAACATGGCGGTG | JrSPL25-F | GCTATGAGTGCTGCGGATTTGCGG | |
JrSPL14-F | GGCTCGTGGTGTGGCAGTTCATC | JrSPL25-R | TTGAAGAGCCCCCGGTCAGAGG | |
JrSPL14-R | CTGAGGGTGTAGGGACTCGGGA | Jractin-F | ATGATGTCAAGGAAGGACTC | |
JrSPL16-F | GGCACCGCAGCGAATGATGACAC | Jractin-R | CACAATGATCTCAGCTCCG |
Fig. 4 Phylogenetic relation of SPL family members of J. regia, A. thaliana, S. lycopersicum and P. trichocarpa Sl: S. lycopersicum; Pt: P. trichocarpa; At: A. thaliana; Jr: J. regia
Fig. 5 Expression analysis of JrSPL genes in different tissues The legend indicates the log2 logarithmic transformation on FPKM value. Red indicates high expression levels and blue indicates low expression levels. S: Stem;L: leaf; M: male flower; F: female flower; E: embryo; K: kernel; I: involucre
Fig. 7 Relative expression of JrSPL gene in J. regia after grafting-induced flowering CK refers to the stem tips of regenerated branches following the grafting of late fruiting walnut trees. Jf1, Jf2, Jf3, Jm, Js1, and Js2 correspondingly denote distinct floral structures within the flowering material of 'Xinxin 2' post-grafting induction, including pure female flowers (Jf1, Jf2), mixed female flowers (Jf3), male flowers (Jm), and stem tips (Js1, Js2). * indicates a significant difference in relative expression levels compared to the control(P < 0.01)
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