Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (7): 183-196.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0078
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DU Zhong-yang1(), YANG Ze1, LIANG Meng-jing1, LIU Yi-zhen1, CUI Hong-li1,2, SHI Da-ming1, XUE Jin-ai1, SUN Yan1, ZHANG Chun-hui1, JI Chun-li1(), LI Run-zhi1()
Received:
2024-01-18
Online:
2024-07-26
Published:
2024-05-24
Contact:
JI Chun-li, LI Run-zhi
E-mail:zhongyang0349@163.com;jichunli@sxau.edu.cn;rli2001@126.com
DU Zhong-yang, YANG Ze, LIANG Meng-jing, LIU Yi-zhen, CUI Hong-li, SHI Da-ming, XUE Jin-ai, SUN Yan, ZHANG Chun-hui, JI Chun-li, LI Run-zhi. Effect of Nano-selenium(SeNPs)in Alleviating Lead Stress and Promoting Growth of Tobacco Seedlings[J]. Biotechnology Bulletin, 2024, 40(7): 183-196.
组别 Group | 1/2 Hoagland | Se(25 mg/L) | SeNPs(25 mg/L) | Pb(100 mg/L) | Pb(200 mg/L) |
---|---|---|---|---|---|
con | + | - | - | - | - |
se | + | + | - | - | - |
SeNPs | + | - | + | - | - |
pb-100 | + | - | - | + | - |
se-pb-100 | + | + | - | + | - |
SeNPs-pb-100 | + | - | + | + | - |
pb-200 | + | - | - | - | + |
se-pb-200 | + | + | - | - | + |
SeNPs-pb-200 | + | - | + | - | + |
Table 1 Experimental treatments for tobacco seedlings
组别 Group | 1/2 Hoagland | Se(25 mg/L) | SeNPs(25 mg/L) | Pb(100 mg/L) | Pb(200 mg/L) |
---|---|---|---|---|---|
con | + | - | - | - | - |
se | + | + | - | - | - |
SeNPs | + | - | + | - | - |
pb-100 | + | - | - | + | - |
se-pb-100 | + | + | - | + | - |
SeNPs-pb-100 | + | - | + | + | - |
pb-200 | + | - | - | - | + |
se-pb-200 | + | + | - | - | + |
SeNPs-pb-200 | + | - | + | - | + |
基因Gene | 引物序列 Primer sequence(5'-3') |
---|---|
NtPOD-F NtPOD-R | TCACTCTACCATCGCCATACTC TGCTGTTCTGCTGTCTCTTCT |
NtCAT-F NtCAT-R | TCTTGCCATTGATGCCAGTTG TCCTGCCTGCTTGAAGTTGT |
NtActin-F NtActin-R | CAAAGCAAGCCTACGCTCT ATACGAATGCCCCCGACT |
Table 2 Primer sequences used in RT-qPCR
基因Gene | 引物序列 Primer sequence(5'-3') |
---|---|
NtPOD-F NtPOD-R | TCACTCTACCATCGCCATACTC TGCTGTTCTGCTGTCTCTTCT |
NtCAT-F NtCAT-R | TCTTGCCATTGATGCCAGTTG TCCTGCCTGCTTGAAGTTGT |
NtActin-F NtActin-R | CAAAGCAAGCCTACGCTCT ATACGAATGCCCCCGACT |
Fig. 1 Phenotype of tobacco seedlings sprayed with Se under different doses of lead stress The superscript in the figure is the name of each treatment listed in Table 1. The same below
组别 Group | 株高 Plant height/cm | 根长 Root length/cm | 总叶片鲜重 Total leaf fresh weight/g | 总叶片干重 Total leaf dry weight/g | 最大叶片鲜重 Maximum leaf fresh weight/g | 最大叶片干重 Maximum leaf dry weight/g | 茎秆鲜重 Stem fresh weight/g | 根鲜重 Root fresh weight/g |
---|---|---|---|---|---|---|---|---|
con | 26.13±0.63a | 18.46±2.99ab | 16.63±0.25a | 0.92±0.08a | 2.56±0.31a | 0.16±0.01a | 6.14±0.24a | 4.05±0.37a |
se | 24.25±0.96b | 20.58±1.24a | 16.16±0.85a | 0.87±0.03a | 2.61±0.3a | 0.16±0.01a | 6.13±0.3a | 4.17±0.09a |
SeNPs | 24.28±0.96b | 20.61±1.24a | 16.18±0.85a | 0.87±0.03a | 2.63±0.3a | 0.16±0.01a | 6.25±0.3a | 4.25±0.09a |
pb-100 | 23.38±2.29bc | 16.04±1.01cd | 14.32±0.39cd | 0.61±0.08bc | 2.53±0.49a | 0.13±0.01b | 5.45±0.49b | 2.69±0.58bc |
se-pb-100 | 23.88±0.25b | 17.06±1.33bc | 15.39±1.13abc | 0.68±0.02b | 2.53±0.26a | 0.13±0.01b | 5.92±0.17a | 2.95±0.28b |
SeNPs-pb-100 | 24.19±0.23b | 17.39±1.33bc | 15.68±1.12ab | 0.69±0.02b | 2.56±0.26a | 0.13±0.01b | 6.03±0.18a | 3±0.28b |
pb-200 | 21.38±1.8d | 14.56±2.14d | 13.95±0.75d | 0.54±0.01c | 2.33±0.3a | 0.12±0.01b | 5.17±0.09b | 1.82±0.61d |
se-pb-200 | 21.88±0.63cd | 17.64±1.15bc | 14.26±1.29cd | 0.66±0.04b | 2.39±0.31a | 0.13±0.01b | 5.21±0.43b | 2.18±0.47cd |
SeNPs-pb-200 | 22.14±0.64cd | 17.94±1.15bc | 14.55±1.29bcd | 0.67±0.04b | 2.42±0.31a | 0.13±0.01b | 5.31±0.43b | 2.22±0.48cd |
Table 3 Effects of Se treatment on the growths of tobacco seedlings under lead stress
组别 Group | 株高 Plant height/cm | 根长 Root length/cm | 总叶片鲜重 Total leaf fresh weight/g | 总叶片干重 Total leaf dry weight/g | 最大叶片鲜重 Maximum leaf fresh weight/g | 最大叶片干重 Maximum leaf dry weight/g | 茎秆鲜重 Stem fresh weight/g | 根鲜重 Root fresh weight/g |
---|---|---|---|---|---|---|---|---|
con | 26.13±0.63a | 18.46±2.99ab | 16.63±0.25a | 0.92±0.08a | 2.56±0.31a | 0.16±0.01a | 6.14±0.24a | 4.05±0.37a |
se | 24.25±0.96b | 20.58±1.24a | 16.16±0.85a | 0.87±0.03a | 2.61±0.3a | 0.16±0.01a | 6.13±0.3a | 4.17±0.09a |
SeNPs | 24.28±0.96b | 20.61±1.24a | 16.18±0.85a | 0.87±0.03a | 2.63±0.3a | 0.16±0.01a | 6.25±0.3a | 4.25±0.09a |
pb-100 | 23.38±2.29bc | 16.04±1.01cd | 14.32±0.39cd | 0.61±0.08bc | 2.53±0.49a | 0.13±0.01b | 5.45±0.49b | 2.69±0.58bc |
se-pb-100 | 23.88±0.25b | 17.06±1.33bc | 15.39±1.13abc | 0.68±0.02b | 2.53±0.26a | 0.13±0.01b | 5.92±0.17a | 2.95±0.28b |
SeNPs-pb-100 | 24.19±0.23b | 17.39±1.33bc | 15.68±1.12ab | 0.69±0.02b | 2.56±0.26a | 0.13±0.01b | 6.03±0.18a | 3±0.28b |
pb-200 | 21.38±1.8d | 14.56±2.14d | 13.95±0.75d | 0.54±0.01c | 2.33±0.3a | 0.12±0.01b | 5.17±0.09b | 1.82±0.61d |
se-pb-200 | 21.88±0.63cd | 17.64±1.15bc | 14.26±1.29cd | 0.66±0.04b | 2.39±0.31a | 0.13±0.01b | 5.21±0.43b | 2.18±0.47cd |
SeNPs-pb-200 | 22.14±0.64cd | 17.94±1.15bc | 14.55±1.29bcd | 0.67±0.04b | 2.42±0.31a | 0.13±0.01b | 5.31±0.43b | 2.22±0.48cd |
Fig. 2 Effects of Se on the chlorophyll and carotenoids in tobacco leaves under lead stress The data were tested by Duncan, and different lowercase letters indicated significant differences between treatments(P<0.05). The same below
组别 Group | 硒含量 Se concentration/(μg·g-1 FW) | 铅含量 Pb concentration/(μg·g-1 FW) | ||
---|---|---|---|---|
叶 Shoot | 根 Root | 叶 Shoot | 根 Root | |
con | - | - | - | - |
se | 307.4±5.53e | 86.4±1.55e | - | - |
SeNPs | 423.99±0.74c | 120.1±1.55c | - | - |
pb-100 | - | - | 88.76±1.37d | 472.02±7.29d |
se-pb-100 | 316.83±3.44d | 86.85±0.97d | 78.83±0.08e | 412.57±1.12e |
SeNPs-pb-100 | 452.3±3.39b | 127.12±0.95b | 69.97±0.91f | 375.75±4.1f |
pb-200 | - | - | 137.44±1.71a | 730.9±9.11a |
se-pb-200 | 313.76±1.69d | 89.85±0.62d | 113.76±1.69b | 612.64±4.77b |
SeNPs-pb-100 | 591.94±1.31a | 172.45±0.37a | 102.2±1.31c | 548.17±1.26c |
Table 4 Effects of Se on the contents of Se and Pb in tobacco seedlings under lead stress
组别 Group | 硒含量 Se concentration/(μg·g-1 FW) | 铅含量 Pb concentration/(μg·g-1 FW) | ||
---|---|---|---|---|
叶 Shoot | 根 Root | 叶 Shoot | 根 Root | |
con | - | - | - | - |
se | 307.4±5.53e | 86.4±1.55e | - | - |
SeNPs | 423.99±0.74c | 120.1±1.55c | - | - |
pb-100 | - | - | 88.76±1.37d | 472.02±7.29d |
se-pb-100 | 316.83±3.44d | 86.85±0.97d | 78.83±0.08e | 412.57±1.12e |
SeNPs-pb-100 | 452.3±3.39b | 127.12±0.95b | 69.97±0.91f | 375.75±4.1f |
pb-200 | - | - | 137.44±1.71a | 730.9±9.11a |
se-pb-200 | 313.76±1.69d | 89.85±0.62d | 113.76±1.69b | 612.64±4.77b |
SeNPs-pb-100 | 591.94±1.31a | 172.45±0.37a | 102.2±1.31c | 548.17±1.26c |
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