Cloning and Expression Analysis of CabZIP42 Gene in Pepper

doi:10.13560/j.cnki.biotech.bull.1985.2024-0465

Abstract

Abstract:

【Objective】To explore the role of bZIP gene in response to abiotic stress would provide genetic resources for improving the stress resistance of peppers. 【Method】A candidate gene CabZIP42 was obtained and cloned based on transcriptome sequencing in pepper ‘G7’, the molecular characteristics of the encoded protein were analyzed by bioinformatics methods, and its expression patterns were analyzed by RT-qPCR in different tissues and under different stress treatments.【Result】The coding region of CabZIP42 was 1 233 bp in length, encoding 410 amino acids, with a predicted molecular weight of 44.85 kD and a theoretical isoelectric point of 9.47. The CabZIP42 protein contained two conserved domains, the basic amino acid region N-x9-R and the leucine zipper region x6-L-x6-L-x6-L. The secondary structure of the protein was dominated by random coils, and contained a small amount of α-helix. Phylogenetic analysis showed that CabZIP42 had the most recent protein evolutionary relationship with tomato and potato. Promoter prediction analysis showed that there were multiple hormonal and stress-related cis-acting elements on the CabZIP42 promoter.【Conclusion】The expression of CabZIP42 gene was the highest in pepper leaves, and it was involved in ABA signal transduction in response to drought, high temperature and high salt stress.

Key words: pepper, bZIP transcription factor, cloning, expression analysis, abiotic stress

LI Bai-xue, LI Jin-ling, CHEN Chun-lin, DU Qing-jie, LI Meng, WANG Ji-qing, MA Yong-bin, XIAO Huai-juan. Cloning and Expression Analysis of CabZIP42 Gene in Pepper[J]. Biotechnology Bulletin, 2024, 40(12): 93-101.

Figures/Tables 8

References 33

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基因 Gene	引物序列 Primer sequence（5'-3'）	用途 Purpose
CabZIP42	F: ATGGGATCTTACATGAACTTC R: CTACCAAGGCCCTGTCAGT	克隆 Cloning
	F: CTCGGCCACTTAGGCAGAAA R: CTAACTACCCCGGCCTTCAC	实时荧光定量 RT-qPCR
CaUBI	F: TGTCCATCTGCTCTCTGTTG R: CACCCCAAGCACAATAAGAC	内参基因 Reference gene

基因 Gene	引物序列 Primer sequence（5'-3'）	用途 Purpose
CabZIP42	F: ATGGGATCTTACATGAACTTC R: CTACCAAGGCCCTGTCAGT	克隆 Cloning
	F: CTCGGCCACTTAGGCAGAAA R: CTAACTACCCCGGCCTTCAC	实时荧光定量 RT-qPCR
CaUBI	F: TGTCCATCTGCTCTCTGTTG R: CACCCCAAGCACAATAAGAC	内参基因 Reference gene

顺式元件类型Cis-acting element	生物学功能Biological function
STRE	防御应激与胁迫响应元件Element involved in defensive stress and stress response
CAAT-box	启动子和增强子Promoter and enhancer
AE-box	光响应Light responsiveness
BOX 4	光响应Light responsiveness
G-box	光响应Light responsiveness
CGTCA-motif	茉莉酸甲酯响应元件Element involved in MeJA responsiveness
TGACG-motif	茉莉酸响应元件Element involved in jasmonic acid responsiveness
ERE	乙烯响应元件Element involved in ethylene responsiveness
TCA	水杨酸响应元件Element involved in SA responsiveness
TCA-element	水杨酸响应元件Element involved in SA responsiveness
as-1	水杨酸响应的顺式作用元件cis-acting element involved in SA responsiveness
MYB	MYB识别和结合元件MYB identifying and binding elements
MYC	MYC识别和结合元件MYC identifying and binding elements
Myb	Myb识别和结合元件MYb identifying and binding elements
CAT-box	分生组织表达相关元件Elements related to meristem expression
F-box	防御应激与胁迫响应元件Element involved in defense stress and stress response
LAMP-element	环境响应元件Element involved in environment responsiveness
TCT-motif	光周期响应元件Element involved in photoperiod responsiveness
TGA-element	植物生长激素响应元件Element involved in plant growth hormone responsiveness
circadian	昼夜节律响应元件Element involved in circadian responsiveness
AAGAA-motif	脱落酸响应元件ABA-responsive element
ABRE	脱落酸响应元件ABA-responsive elements
GATA-motif	光响应Light responsiveness
GT1-motif	光响应Light responsiveness
TC-rich repeats	防御应激与胁迫响应元件Element involved in defensive stress and stress response
TATA-box	转录起始-30左右的核心启动子元件Core promoter element around -30 of transcription start

顺式元件类型Cis-acting element	生物学功能Biological function
STRE	防御应激与胁迫响应元件Element involved in defensive stress and stress response
CAAT-box	启动子和增强子Promoter and enhancer
AE-box	光响应Light responsiveness
BOX 4	光响应Light responsiveness
G-box	光响应Light responsiveness
CGTCA-motif	茉莉酸甲酯响应元件Element involved in MeJA responsiveness
TGACG-motif	茉莉酸响应元件Element involved in jasmonic acid responsiveness
ERE	乙烯响应元件Element involved in ethylene responsiveness
TCA	水杨酸响应元件Element involved in SA responsiveness
TCA-element	水杨酸响应元件Element involved in SA responsiveness
as-1	水杨酸响应的顺式作用元件cis-acting element involved in SA responsiveness
MYB	MYB识别和结合元件MYB identifying and binding elements
MYC	MYC识别和结合元件MYC identifying and binding elements
Myb	Myb识别和结合元件MYb identifying and binding elements
CAT-box	分生组织表达相关元件Elements related to meristem expression
F-box	防御应激与胁迫响应元件Element involved in defense stress and stress response
LAMP-element	环境响应元件Element involved in environment responsiveness
TCT-motif	光周期响应元件Element involved in photoperiod responsiveness
TGA-element	植物生长激素响应元件Element involved in plant growth hormone responsiveness
circadian	昼夜节律响应元件Element involved in circadian responsiveness
AAGAA-motif	脱落酸响应元件ABA-responsive element
ABRE	脱落酸响应元件ABA-responsive elements
GATA-motif	光响应Light responsiveness
GT1-motif	光响应Light responsiveness
TC-rich repeats	防御应激与胁迫响应元件Element involved in defensive stress and stress response
TATA-box	转录起始-30左右的核心启动子元件Core promoter element around -30 of transcription start