Effects of Co-treatment of Nano-silica and Bacillus cereus SS1 on the Growth of Tobacco

doi:10.13560/j.cnki.biotech.bull.1985.2025-0089

Abstract

Abstract:

Objective A growth-promoting Bacillus cereus strain SS1 was identified previously in our laboratory. The objective of this study is to examine the combined effects of silicon nanopowder and B. cereus SS1 on the growth of tobacco, as well as to explore the related functional mechanism. Method Zhongyan 101 was used as experimental material. Tobacco seedlings were treated with silicon nanopowder and SS1 alone or in combination, the growth and photosynthetic indexes, as well as the activities and gene expressions of nitrogen metabolism-related enzymes of each treatment were detected. Result The nanometer silica and SS1 at certain concentration, and co-treatment of nanopowder and SS1 all promoted the growth of tobacco, and the promotion effect of co-treatment was the most significant. The above-ground growth indexes of co-treated tobacco, such as plant height, stem diameter, leaf number, and leaf area, were higher than those of the seedlings treated with silicon nanopowder or SS1 alone and the non-treated control plants; moreover, the root length, root weight and root-shoot ratio also significantly increased by co-application. Meanwhile, the results showed that the co-treatment of SS1 and silicon nanopowder significantly increased the chlorophyll content, net photosynthetic rate, stomatal conductance and other photosynthetic indexes of tobacco plants. The activities of nitrate reductase (NR) and glutamine synthetase (GR) as well as the expressions of related genes NtNIA1 and NtGS1 also more significantly increased by SS1 and silicon nanopowder co-treatment compared to treatment with SS1 or silicon nanopowder alone. Conclusion The combination of silicon nanopowder and SS1 may promote plant growth by ameliorating the photosynthetic performance and promoting nitrogen metabolism of plant.

Key words: nano-silica, Bacillus cereus, nitrogen metabolism, growth, tobacco

DONG Xu-kun, CHE Yong-mei, WANG Ming-shuo, LUO Zheng-gang, GUAN En-sen, ZHAO Fang-gui, YE Qing, LIU Xin. Effects of Co-treatment of Nano-silica and Bacillus cereus SS1 on the Growth of Tobacco[J]. Biotechnology Bulletin, 2025, 41(7): 292-298.

Figures/Tables 5

Table 1 Primer sequences used in RT-qPCR

引物名称 Primer name	引物序列 Primer sequence （5′-3′）
qRT-NtActin-F	GAAGAAGGTCCCAAGGGTTC
qRT-NtActin-R	TCTCCCTTTAACACCAACGG
qRT-NtNIA1-F	GTGTGGCCCTCATTCCAAGA
qRT-NtNIA1-R	CGTGCTAGTAGGCGTGTAGG
qRT-NtGS1-F	GGGTTTGGCATACATTCTTG
qRT-NtGS1-R	GTGTCTTCTTCACTGGGTGG

Fig. 1 Effects of silicon nanopowder (Si) and SS1 co-treatment on the plant height, stem diameter, leaf number, and leaf area of tobaccoThe different letters on the column in the figure indicate significant differences between different treatments (P<0.05). The same below

Fig. 2 Effects of co-treatment of silicon nanopowder with SS1 on the root length, root dry weight and root-shoot ratio of tobacco seedling

Fig. 3 Effects of co-treatment of silicon nanopowder with SS1 on the chlorophyll content, net photosynthetic rate, stomatal conductance, transpiration rate, water use efficiency and intercellular carbon dioxide concentration of tobacco leaves

Fig. 4 Effects of co-treatment with silicon nanopowder and SS1 on nitrogen metabolism-related enzyme activities and genes expression in tobacco

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