Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (1): 203-208.

• Research report • Previous Articles    

Determination Methods for Lipase Activity and Its Application in the Screening of Microbial Lipase

Wang Huan1,2,He Laping3,4,Zhou Huanjing1,2,Zhang Yiming1, Li Cuiqin2, Tao Han1,2   

  1. 1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy，Guiyang 550025;
    2. School of Chemistry and Chemical Engineering，Guizhou University，Guiyang 550025;
    3.College of Life Sciences，Guizhou University，Guiyang 550025 ；4. Key Laboratory of Agricultural and Animal Products Store & Processing of Guizhou Province，Guiyang 550025
  • Received:2012-10-29 Revised:2013-01-31 Online:2013-01-30 Published:2013-01-30

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Abstract: Four common lipase activity determination methods including Rhodamine B plate, olive oil emulsification, ρ-NPP for lipasehydrolytic activity and ρ-NPP for lipase synthetic activity were compared. The result showed that the Rhodamine B plate method was suitable forthe qualitative and preliminary quantitative estimate of lipase activity and the other three methods were suitable for the quantitative determinationof lipase activity but only the ρ-NPP method has a good reproducibility. Moreover, there is no relationship between the hydrolytic activity andsynthetic activity of lipase. So lipase activity determination method should be chosen in screening the lipase-produced microbial. Namely,hydrolytic activity determination methods should be chosen in screening the hydrolytic lipase-produced microbial, otherwise, ester synthetaseactivity assay should be used. Based on the principle, desired lipase-produced microbial were screened.

Key words: Lipase , Hydrolytic activity , Synthetic activity , Determination methods , Screening