Genetic Transformation of Bt（cry1Ab）Gene into Sugarcane（Saccharum officinarum L.）Mediated by Agrobacterium tumefaciens

Abstract

Abstract: In this research, embryonic callus induced from sugarcane（Saccharum officinarum L.）cultivar “Chuanzhe 23” was selected as recipient material. We successfully transformed anti-insect gene Bt（cry1Ab）into sugarcane mediated by Agrobactenum tumefaciens EHA105 when the explants infected with lower concentration of Agrobactenum tumefaciens（OD ≈ 0.1）and shorter infusion time（1.5 min）. The optimal concentrations of hygromycin for the selection of transformed shoots and roots induction respectively were 20 mg/L and 30 mg/L. The calli cultured for 40 d were optimal for transformation; 65 transformants were obtained after successive hygromycin resistance selection. The hyg-resistant plants were detected by PCR analysis, and 2 plants showed positive, which initially proved that the target gene had been integrated into sugarcane genome．

Key words: Sugarcane（Saccharum officinarum L.）, Genetic transformation, Bt（cry1Ab）gene

Li Xiaomei Wang Minxia Qin Tinghao Yang Cui An Qi Zhang Jun. Genetic Transformation of Bt（cry1Ab）Gene into Sugarcane（Saccharum officinarum L.）Mediated by Agrobacterium tumefaciens[J]. Biotechnology Bulletin, 2013, 0(2): 100-105.

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